PXD064066

PXD064066 is an original dataset announced via ProteomeXchange.

Title	Rapid expansion of primary human vocal fold epithelial cells via targeted pathway inhibition and anchorage-independent sphere culture
Description	Vocal fold epithelial cells (VFE) serve critical physiologic and immunologic functions at the boundary between the upper and lower airways but are difficult to maintain and expand in primary culture. This technical challenge has impeded progress in VFE biology as well as cell banking for translational applications. Here, using primary human VFE, we show that simultaneous inhibition of TGF-β, ROCK, and Notch signaling with a small-molecule inhibitor cocktail enables rapid proliferation, successful passaging, and long-term expansion while preserving core epithelial phenotype. Under anchorage-independent culture conditions, VFE progenitors generate clonal spheres that can be expanded over multiple generations; sphere-dissociated VFE then revert towards their original phenotype, including the ability to form stratified squamous epithelium in organotypic coculture. Both pathway-inhibited and sphere-cultured VFE exhibit mechanistically appropriate remodeling of the cellular proteome. These advances offer a robust toolkit for upper airway mucosal biology and regenerative medicine.
HostingRepository	PRIDE
AnnounceDate	2025-11-06
AnnouncementXML	Submission_2025-11-06_12:57:51.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiyan Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-19 12:03:50	ID requested
⏵ 1	2025-11-06 12:57:51	announced

Dataset with its publication pending

submitter keyword: None

Lingjun Li
contact affiliation	2Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, Madison, WI 53705, USA
contact email	lingjun.li@wisc.edu
lab head
Haiyan Lu
contact affiliation	University of Wisconsin-Madison
contact email	luhaiyan2017@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD064066

PRIDE project URI

• PRIDE
  1. PXD064066
     1. Label: PRIDE project
     2. Name: Rapid expansion of primary human vocal fold epithelial cells via targeted pathway inhibition and anchorage-independent sphere culture