PXD064064

PXD064064 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic profiling of lipopolysaccharide stimulated toll-like receptor pathways in immortalized mouse macrophages
Description	Toll-like receptors (TLRs) are present on the membranes of immune cells such as dendritic cells, macrophages, and natural killer cells. It is essential for the recognition of microbiological components. They trigger both innate and adaptive immune responses against pathogenic microorganisms. Among TLRs, TLR4 is one of the well-studied receptors that recognizes lipopolysaccharide (LPS) and initiates the TRIF- and MyD88-dependent signaling cascades. Regulators in TLR4 signaling, including protein kinases, play a key role in innate and adaptive immune responses. Protein phosphorylation on serine, threonine, and tyrosine is essential regulatory post-translational modification (PTM) because it serves as a signal transduction mechanism enabling the cells to connect extracellular signals to the regulation of multiple biological processes. Although phosphoproteins in TLR4 signaling pathways have been extensively investigated by mass spectrometry using data-dependent acquisition (DDA), the phosphoproteins that initiate TLR4 signaling cascades remain poorly understood due to technical limitations. We utilized data-independent acquisition (DIA) to get comprehensive information on phosphorylation inside LPS-stimulated TLR4 signaling pathways in macrophages.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_15:31:07.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-19 10:36:46	ID requested
⏵ 1	2025-09-30 15:31:08	announced

Dataset with its publication pending

submitter keyword: TLR4, LPS, Data independent acquisition,Phosphoproteomics

Aleksandra Nita-Lazar
contact affiliation	Functional Cellular Networks Section, Laboratory of Immune System Biology, NIAID, NIH
contact email	nitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064064
     1. Label: PRIDE project
     2. Name: Phosphoproteomic profiling of lipopolysaccharide stimulated toll-like receptor pathways in immortalized mouse macrophages