PXD064050

PXD064050 is an original dataset announced via ProteomeXchange.

Title	PARN immunoprecipitates with CPSF6, in NCI-H520 Cells
Description	The previous results suggest that PARN is involved in the early steps of miR-29a maturation. To identify factors that associate with and could recruit PARN to polyadenylated pri-miR-29a transcripts, we performed immunoprecipitation experiments in extracts of NCI-H520 cells with PARN antibody, followed by mass spectrometry. Of the identified proteins, we focused on cleavage and polyadenylation specificity factor 6 (CPSF6), the 68 kDa larger component of the mammalian cleavage factor I (CFIm), which is essential for the early steps of mRNA 3 ́ end processing and the selection of multiple poly(A) sites
HostingRepository	PRIDE
AnnounceDate	2026-03-07
AnnouncementXML	Submission_2026-03-07_11:17:51.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-19 02:44:12	ID requested
⏵ 1	2026-03-07 11:17:52	announced

Dataset with its publication pending

submitter keyword: CPSG6, In-gel digestion, IP,PARN

Nikolaos A. A. Balatsos
contact affiliation	Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
contact email	balatsos@bio.uth.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064050

PRIDE project URI

• PRIDE
  1. PXD064050
     1. Label: PRIDE project
     2. Name: PARN immunoprecipitates with CPSF6, in NCI-H520 Cells