PXD064018

PXD064018 is an original dataset announced via ProteomeXchange.

Title	Combination of in vivo and in vitro phosphoproteomics determines the PP2A target repertoire on proteome scale
Description	Dynamics of protein phosphorylation are regulated by the interplay of protein kinases and phosphatases. Current mass spectrometry-based phosphoproteomic approaches are extremely powerful in identifying and quantifying tens of thousands of different phosphorylation events in single biological samples. However, whereas the mapping of phosphorylation events has been successfully automated supporting high sample throughput, the characterization of responsible kinases and phosphatases still largely depends on laborious protein biochemical assays. To show direct (de)phosphorylation events in vitro kinase or phosphatase assays using single substrates or peptide arrays are still the methods of choice. Here we describe the development of an in vitro phosphatase assay using whole proteome under native conditions as input. We employ this approach to study the PP1 and PP2A target repertoire, characterizing thousands of potential target sites. Focusing on PPP2R5E/B56-containing complexes, we combine in vitro with in vivo phosphoproteomics to characterize bona fide target sites, which highlight PP2A`s role in regulating stress granule assembly.
HostingRepository	PanoramaPublic
AnnounceDate	2025-05-16
AnnouncementXML	Submission_2025-05-16_09:38:14.967.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Melanie Brunner
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Phospho
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-16 09:25:57	ID requested
⏵ 1	2025-05-16 09:38:15	announced

Dataset with its publication pending

submitter keyword: Phosphatase, PRM, OBIPhA

Joern Dengjel
contact affiliation	University of Fribourg
contact email	joern.dengjel@unifr.ch
lab head
Melanie Brunner
contact affiliation	University of Fribourg
contact email	melanie.brunner@unifr.ch
dataset submitter

Panorama Public dataset URI