PXD064001 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of proteins immunoprecipitated with anti-FLAG antibodies from Leishmania donovani promastigotes expressing Cdc45-FLAG or carrying empty vector |
| Description | This study was carried out to identify the interacting partners of Leishmania donovani Cdc45, a protein that is conserved in eukaryotic DNA replication. Accordingly, Cdc45 was expressed in Leishmania promastigotes with a C terminal FLAG tag and whole cell lysates were isolated from these Ld1S transfectant promastigotes. Whole cell lysates were also isolated from promastigotes harbouring empty vector. These lysates were used in immunoprecipitation reactions with anti-FLAG antibodies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-27 |
| AnnouncementXML | Submission_2025-10-26_17:33:30.722.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Swati Saha |
| SpeciesList | scientific name: Leishmania donovani; NCBI TaxID: NEWT:5661; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-15 22:52:01 | ID requested | |
| ⏵ 1 | 2025-10-26 17:33:31 | announced | |
Publication List
| Dashora V, Singh A, Pandey A, Saha S, Identification of Cdc45-interacting partners uncovers two Leishmania donovani proteins involved in DNA replication. BMC Microbiol, 25(1):675(2025) [pubmed] |
| 10.1186/s12866-025-04377-7; |
Keyword List
| submitter keyword: LC-MS,Leishmania donovani, Immunoprecipitation, Cdc45-FLAG |
Contact List
| Professor Swati Saha |
|---|
| contact affiliation | Department of Microbiology, University of Delhi South Campus Benito Juarez Marg, South Campus, South Moti Bagh, New Delhi, Delhi 110021 India |
| contact email | ssaha@south.du.ac.in |
| lab head | |
| Swati Saha |
|---|
| contact affiliation | Department of Microbiology, University of Delhi South Campus, New Delhi |
| contact email | swatisahalab@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD064001 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064001
- Label: PRIDE project
- Name: Analysis of proteins immunoprecipitated with anti-FLAG antibodies from Leishmania donovani promastigotes expressing Cdc45-FLAG or carrying empty vector
