PXD063958 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Analysis of Wharton’s Jelly Mesenchymal Stem Cells Isolated by Different Methods |
| Description | Wharton’s Jelly mesenchymal stem cells (WJ-MSC) are stem cells from the umbilical cord, known for their regenerative properties. They offer advantages like unlimited availability, non-invasive and low-cost isolation, and minimal ethical concerns. WJ-MSCs are promising for allogeneic cell therapy, with clinical trials showing their safety and efficacy across various diseases. They are also considered non-tumorigenic. A critical step for clinical use is selecting the optimal isolation method. This study compares three techniques: two explant methods (with and without scraping) and one enzymatic method. Additionally, the effect of adding fibroblast growth factor 2 (FGF2) to enhance cell expansion is examined. While several studies have addressed differences in isolation methods, the impact on the protein profile of WJ-MSCs is less explored. Therefore, we aim to explore the proteomic profiles of WJ-MSCs isolated by different methods to better understand their mechanisms and functionalities |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-29_16:32:17.387.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063958 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Niyaz Alsharabi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-15 01:28:43 | ID requested | |
| ⏵ 1 | 2026-03-29 16:32:18 | announced | |
Publication List
| 10.6019/PXD063958; |
| 10.1371/journal.pone.0345081; |
| Nguyen JL, Mohamed-Ahmed S, Saleem R, Mustafa K, Al-Sharabi N, Bentsen MHL, Isolation methods influence the biological properties of Wharton's Jelly-derived mesenchymal stem cells: A comparative study of yield, viability, proliferation, differentiation potential, and proteomic profiles. PLoS One, 21(3):e0345081(2026) [pubmed] |
Keyword List
| submitter keyword: Wharton’s Jelly Mesenchymal Stem Cells, Proteomic, isolation methods |
Contact List
| kamal Mustafa |
|---|
| contact affiliation | Head of TIssue Engineering Group at the Departmnet of Dentistry |
| contact email | kamal.Mustafa@uib.no |
| lab head | |
| Niyaz Alsharabi |
|---|
| contact affiliation | University of Bergen |
| contact email | n.al-sharabi@uib.no |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD063958 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063958
- Label: PRIDE project
- Name: Proteomic Analysis of Wharton’s Jelly Mesenchymal Stem Cells Isolated by Different Methods
