PXD063956 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | De novo designed protein guiding targeted protein degradation |
Description | Targeted protein degradation is a powerful tool for biological research, cell therapy, and synthetic biology. However, conventional methods often depend on pre-fused degrons or chemical degraders, limiting their wider applications. In this study, a guided protein labeling and degradation system (GPlad) was developed in Escherichia coli, using de novo designed guide proteins and arginine kinase (McsB) for precise degradation of various proteins, including fluorescent proteins, metabolic enzymes, and human proteins. GPlad was expanded into versatile tools such as antiGPlad, OptoGPlad, and GPTAC, enabling reversible inhibition, optogenetic regulation, and biological chimerization. The combination of GPlad and antiGPlad allows for programmable circuit construction, such as ON/OFF switches, signal amplifiers, and oscillators. OptoGPlad-mediated degradation of MutH accelerated E. coli evolution under protocatechuic acid stress, reducing the required generations from 220 to 100. Additionally, GPTAC-mediated degradation of AroE enhanced the titer of 3-dehydroshikimic acid to 92.6 g/L, a 23.8% improvement over the conventional CRISPR interference method. GPlad provides a tunable, plug-and-play strategy for straightforward protein degradation without the need for pre-fusion, with substantial implications for synthetic biology and metabolic engineering |
HostingRepository | PRIDE |
AnnounceDate | 2025-07-05 |
AnnouncementXML | Submission_2025-07-04_22:34:38.423.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | zhendong Li |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-05-15 00:55:49 | ID requested | |
⏵ 1 | 2025-07-04 22:34:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Protein degradation |
Protein design |
Contact List
Zhendong Li |
contact affiliation | Jiangnan university |
contact email | 7200201049@stu.jiangnan.edu.cn |
lab head | |
zhendong Li |
contact affiliation | Jiangnan university |
contact email | 7200201049@stu.jiangnan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063956 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063956
- Label: PRIDE project
- Name: De novo designed protein guiding targeted protein degradation