PXD063952

PXD063952 is an original dataset announced via ProteomeXchange.

Title	Unveiling Proteomic Responses to Combined Nisin and Urolithin B Treatment (7:3) in Lymphoma Cells
Description	This study investigates the antiproliferative and pro-apoptotic effects of the postbiotics nisin and urolithin B (UB) on lymphoma cells, individually and in combination. Human Burkitt lymphoma (HKB-11) and Hs 313.T cell lines and normal stromal HS-5 cells were treated with nisin, UB, and nine different ratios of their combinations. A 7:3 ratio (nisin 5600 μM + UB 150 μM) demonstrated synergistic anticancer activity with minimal cytotoxicity to normal cells. Flow cytometry confirmed that UB alone was the most potent inducer of apoptosis (p < 0.0001), followed by the 7:3 combination, significantly improving nisin’s apoptotic effect. Reactive oxygen species (ROS) assays demonstrated that nisin triggered concentration-dependent ROS production, whereas UB modulated this effect when combined, thereby reducing oxidative stress. Label-free proteomic analysis revealed distinct expression profiles. Nisin treatment upregulated oxidative stress-related proteins (HBA1, CYC1) and suppressed cell cycle proteins (CDK1, CCNB1), indicating mitotic inhibition. UB altered mitochondrial and epigenetic regulators (CYCS, DNMT1), and the combination therapy significantly downregulated ribosomal proteins (RPS14, RPL11, RPS27) and TP53, suggesting that ribosomal stress mediates antiproliferative effects. Synergy analysis confirmed combination index (CI) values <1 at 4:6 and 7:3 ratios, validating synergistic interactions.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_15:00:55.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Alsherbiny
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-14 23:22:31	ID requested
⏵ 1	2025-07-30 15:00:55	announced

10.3390/IJMS26157369;

submitter keyword: Proteomics, postbiotics, Lymphoma

Deep Bhuyan
contact affiliation	NICM Health Research Institute, Western Sydney University
contact email	D.Bhuyan@westernsydney.edu.au
lab head
Muhammad Alsherbiny
contact affiliation	1) Freedman Foundation Metabolomics Facility, Innovation Centre, Victor Chang Cardiac Research Institute UNSW 2) Pharmacognosy Department, Faculty of Pharmacy, Cairo University
contact email	muhammad.alsherbiny@pharma.cu.edu.eg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063952

PRIDE project URI

• PRIDE
  1. PXD063952
     1. Label: PRIDE project
     2. Name: Unveiling Proteomic Responses to Combined Nisin and Urolithin B Treatment (7:3) in Lymphoma Cells