PXD063948

PXD063948 is an original dataset announced via ProteomeXchange.

Title	Gut microbial postbiotics as potential therapeutics for lymphoma: Proteomics Insights of the synergistic effects of Nisin and Urolithin B against human lymphoma cells
Description	Lymphoma remains a substantial global health concern, necessitating innovative therapeutic strategies. The emerging role of gut microbiota-derived metabolites, known as postbiotics, offers promising avenues for cancer treatment. This study evaluated the antiproliferative activities of various postbiotics, including Nisin (N) and urolithin B (UB), and their combinations against the human lymphoma cell line HKB-11. Systematically, the antiproliferative effects and underlying mechanisms were characterised using Alamar Blue assays, combination index (CI) analyses, ROS measurements, flow cytometry, and bottom-up proteomics analyses of combined and mono-treatments. Nisin and UB demonstrated significant antiproliferative activity with IC50 values of 1467 µM and 87.56 µM, respectively. Among other ratios, their combination at a ratio of 4:6 exhibited potent synergy (CI =0.09 at IC95), notably enhancing apoptosis (p < 0.0001) and modulating ROS levels. Proteomics analyses identified key protein alterations involved in lipid metabolism, mitochondrial function, cell cycle progression, and apoptosis.
HostingRepository	PRIDE
AnnounceDate	2025-07-19
AnnouncementXML	Submission_2025-07-18_17:01:23.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Alsherbiny
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-14 22:14:38	ID requested
⏵ 1	2025-07-18 17:01:24	announced

10.3390/IJMS26146829;

submitter keyword: Proteomics, postbiotics, Lymphoma

Deep Bhuyan
contact affiliation	NICM Health Research Institute, Western Sydney University
contact email	D.Bhuyan@westernsydney.edu.au
lab head
Muhammad Alsherbiny
contact affiliation	1) Freedman Foundation Metabolomics Facility, Innovation Centre, Victor Chang Cardiac Research Institute UNSW 2) Pharmacognosy Department, Faculty of Pharmacy, Cairo University
contact email	muhammad.alsherbiny@pharma.cu.edu.eg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063948

PRIDE project URI

• PRIDE
  1. PXD063948
     1. Label: PRIDE project
     2. Name: Gut microbial postbiotics as potential therapeutics for lymphoma: Proteomics Insights of the synergistic effects of Nisin and Urolithin B against human lymphoma cells