PXD063936

PXD063936 is an original dataset announced via ProteomeXchange.

Title	Altered electron flow in hydrogenotrophic methanogens under nickel limitation
Description	In the methanogenic pathway from CO2 and H2, low potential electrons for CO2 reduction are generated by a flavin-based electron branching reaction catalysed by heterodisulphide reductase (Hdr) in complex with [NiFe]-hydrogenase (Mvh). The F420-reducing [NiFe]-hydrogenase (Frh) provides electrons for the methane formation pathway via the electron carrier F420. The production of both [NiFe]-hydrogenases in Methanothermobacter marburgensis is strongly down-regulated under strictly nickel-limited conditions. The Frh reaction is replaced by a coupled reaction with [Fe]-hydrogenase (Hmd), and the role of Mvh is taken over by F420-dependent electron donating proteins (Elp). Thus, Hmd provides all electrons for the reducing metabolism under these nickel-limited conditions.
HostingRepository	PRIDE
AnnounceDate	2025-08-18
AnnouncementXML	Submission_2025-08-18_02:51:06.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Methanothermococcus thermolithotrophicus; NCBI TaxID: 2186; scientific name: Methanothermobacter marburgensis; NCBI TaxID: 145263;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-14 14:00:19	ID requested
⏵ 1	2025-08-18 02:51:06	announced

Dataset with its publication pending

submitter keyword: electron-bifurcation electron-bifurcation, [NiFe]-hydrogenase, Methanothermobacter marburgensis

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063936

PRIDE project URI

• PRIDE
  1. PXD063936
     1. Label: PRIDE project
     2. Name: Altered electron flow in hydrogenotrophic methanogens under nickel limitation