PXD063924

PXD063924 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Unveiling Proteomic and Peptide-Level Modifications in Cerebrospinal Fluid and Plasma in Normal Cognitive Aging
Description	This study presents a comprehensive proteomic and peptide level analysis of matched cerebrospinal fluid (CSF) and plasma samples from cognitively normal young (n=52) and older (n=40) adults, employing mass spectrometry to elucidate protein-level and peptide-level changes associated with normal cognitive aging. Differential abundance analysis revealed significant upregulation of extracellular matrix components (ECM), coagulation, and inflammatory pathways in CSF and downregulation of insulin-like growth factor-1 (IGF-1) signaling pathway in plasma with aging based on gene set enrichment and weighted correlation network analyses. Novel alternative protein cleavage/splicing and phosphorylation patterns were identified in many proteins involved in lipid metabolism, ECM structure, axonogenesis and synaptic activity such as APP, APOE, COL4A2, NRXN1, and NRCAM. The examination of alternative cleavage/splicing and phosphorylation events in CSF and plasma provided unprecedented insights into age-associated protein modifications. These findings offer potential biomarkers and therapeutic targets and highlight the complex molecular landscape of normal cognitive aging.
HostingRepository	MassIVE
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-05-14_11:07:50.109.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roland Bruderer
SpeciesList	scientific name: homo;
ModificationList	Oxidation; CarbamidomethylDTT
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-14 04:58:18	ID requested
⏵ 1	2025-05-14 11:07:50	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: cerebrospinal fluid, plasma, normal cognitive aging, DIA mass spectrometry, data-independent acquisition, deep proteomics, peptide-level analysis, alternative cleavage, alternative splicing, DatasetType:Proteomics

submitter keyword: cerebrospinal fluid, plasma, normal cognitive aging, DIA mass spectrometry, data-independent acquisition, deep proteomics, peptide-level analysis, alternative cleavage, alternative splicing, DatasetType:Proteomics

Contact List

Roland Bruderer
contact affiliation	Biognosys AG
contact email	roland.bruderer@biognosys.com
lab head
Abhay Moghekar
contact affiliation	Johns Hopkins University
contact email	m@jhmi.edu
lab head
Roland Bruderer
contact affiliation	Biognosys AG
contact email	roland.bruderer@biognosys.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097888/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097888/