PXD063910

PXD063910 is an original dataset announced via ProteomeXchange.

Title	Haptoglobin and glutamine synthetase as potential biomarkers of chemotherapy-induced cachexia in skeletal muscle
Description	Cancer cachexia is a multifactorial wasting syndrome affecting body and lean tissue mass that is often exacerbated by anticancer chemotherapy. In this study, we used a mouse model of acute myeloid leukemia chemotherapy induction regimen (CIR) comprising daunorubicin and cytarabine to investigate the molecular mechanisms underlying cachexia. Quantitative tandem mass tag (TMT) based proteomics was performed on skeletal muscle (quadriceps) to uncover biomarkers and pathways associated with chemotherapy induced muscle wasting. Our findings demonstrate that the AML CIR induced an acute cachexic phenotype characterized by approximately 10 percent body and lean mass loss and 20 percent muscle fibre atrophy. Through deep proteome profiling, two potential biomarkers—haptoglobin (Hp) and glutamine synthetase (Glul)—were identified. Haptoglobin in particular was responsive to cachexia severity, recovery, and exacerbation via exercise, indicating its potential as a conditionally sensitive biomarker of muscle wasting. Pathway analysis revealed upregulation of mitochondrial metabolism including branched chain amino acid catabolism and mitochondrial uncoupling proteins (Ucp1 and Ucp3), suggesting hypermetabolism as a key driver of the phenotype. These data support the use of skeletal muscle proteomics in characterizing chemotherapy induced cachexia and identifying sensitive muscle specific biomarkers for future translational and therapeutic studies.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:15:25.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Steele
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-13 20:37:05	ID requested
⏵ 1	2025-06-15 16:15:26	announced

10.1002/jcsm.13849;

Campelj DG, Timpani CA, Spiesberger G, Formosa LE, Steele JR, Zhang H, Schittenhelm RB, Leow L, Goodman CA, Rybalka E, Haptoglobin and Glutamine Synthetase May Biomark Cachexia Induced by Antiacute Myeloid Leukaemia Chemotherapy. J Cachexia Sarcopenia Muscle, 16(3):e13849(2025) [pubmed]

submitter keyword: Cancer cachexia, proteomics, skeletal muscle wasting, glutamine synthetase, TMT, mitochondrial metabolism, acute myeloid leukemia, haptoglobin, chemotherapy, biomarkers, AML

Dr. Emma Rybalka
contact affiliation	Institute for Health and Sport, Victoria University St Albans, Victoria 3021, Australia
contact email	emma.rybalka@vu.edu.au
lab head
Joel Steele
contact affiliation	Monash
contact email	joel.steele@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD063910

PRIDE project URI

• PRIDE
  1. PXD063910
     1. Label: PRIDE project
     2. Name: Haptoglobin and glutamine synthetase as potential biomarkers of chemotherapy-induced cachexia in skeletal muscle