PXD063904

PXD063904 is an original dataset announced via ProteomeXchange.

Title	Untargeted Urinary Proteomics Uncovers Nephroprotective and Systemic Adaptations after Obesity Surgery Induced Weight Loss
Description	Weight loss induced by bariatric surgery (BS) is recognized for having a profound impact on several biological systems. This study aimed to characterize the urinary proteomic profile in individuals with obesity before and after BS, to identify urinary proteins that reflect the kidney and systemic adaptations to weight loss. Urine samples from individuals with obesity (n=16) undergoing BS were collected before and two-years after surgery. Untargeted high-resolution liquid chromatography–mass spectrometry (LC-MS) with label-free quantification (LFQ) was used to assess changes in the urinary proteome. A total of 2347 proteins were identified in the urine. Among these, 1016 depicted a significantly different expression post-surgery (p<0.05). In particular, 54 proteins were identified with at least 50% change (≥1.5-fold), either upregulated (n=42) or downregulated (n=12). Functional classification of these top altered proteins revealed an association with immune function (n=17; e.g., protein S100-A9, alpha-1-acid glycoproteins 1 and 2); cytoskeleton/cell adhesion (n=11; e.g., supervillin, ezrin, periplakin) and kidney adaptation (n=11; e.g., elongation factor 1-alpha 1, cystatin-B, megalin, cubilin). A decrease in inflammation protein markers (alpha-1-acid glycoproteins), alongside an increase in proteins associated with immune modulation and oxidative stress protection (dipeptidase 1 and heat shock cognate 71 kDa protein) were also observed post-surgery. Additionally, changes in cytoskeletal and extracellular matrix proteins were identified, as well as proteins present in specific kidney cell components and tubular processes. The most significant modifications found in the urinary proteome following BS were proteins primarily associated with decreased inflammation and oxidative stress, as well as cytoskeletal and extracellular matrix proteins. Overall, our results reveal potential novel pathways contributing to systemic modifications and nephroprotective effects of BS induced weight loss.
HostingRepository	PRIDE
AnnounceDate	2025-12-10
AnnouncementXML	Submission_2025-12-10_13:49:37.386.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063904
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Carrageta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-13 18:44:10	ID requested
⏵ 1	2025-12-10 13:49:37	announced

10.1021/ACS.JPROTEOME.5C00500;

10.6019/PXD063904;

submitter keyword: bariatric interventions, obesity surgery, obesity-related kidney disease, mass spectrometry,obesity, urine proteomics

Mariana Pereira
contact affiliation	Unit for Multidisciplinary Research in Biomedicine (UMIB), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, Porto, Portugal
contact email	mpmonteiro@icbas.up.pt
lab head
David Carrageta
contact affiliation	Unit for Multidisciplinary Research in Biomedicine (UMIB), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, Porto, Portugal
contact email	djcarrageta@icbas.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD063904

PRIDE project URI

• PRIDE
  1. PXD063904
     1. Label: PRIDE project
     2. Name: Untargeted Urinary Proteomics Uncovers Nephroprotective and Systemic Adaptations after Obesity Surgery Induced Weight Loss