PXD063898 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative proteomics of small extracellular vesicles from lymphatic fluid for analyzing the premetastatic niche in melanoma |
| Description | Melanoma is an aggressive form of skin cancer that often spreads via lymphatic pathways to regional and distant sites. Melanoma-derived lymphatic small extracellular vesicles (sEVs) play a crucial role in forming a tumor-supportive environment for metastasis, or premetastatic niche, within the first tumor draining lymph node, also known as the sentinel lymph node (SLN). Therefore, analyzing the proteomic content of tumor-draining lymphatic sEVs that deliver oncogenic molecules to the SLN is important in understanding the premetastatic niche. To reveal the proteomic landscape of lymphatic sEVs we performed multidimension liquid chromatography-tandem mass spectrometry with multiplexing (18-samples) using tandem mass tag (TMT) pro labeling to profile the lymphatic sEVs proteomes obtained from afferent lymphatic channels leading to the SLN of patients with melanoma (n=6), control afferent lymphatic channels from prophylactic mastectomy (n=3) and non-cancer post-operative lymphatic fluid (n=9). Lymphatic fluid from postoperative lymphadenectomy drains served as another control to filter out non-melanoma alteration in lymph that may be related to the procedure of surgical resection and wound healing process. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-19 |
| AnnouncementXML | Submission_2026-01-18_16:28:36.674.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063898 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Shankar Suman |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-13 14:03:10 | ID requested | |
| ⏵ 1 | 2026-01-18 16:28:37 | announced | |
Publication List
| 10.1016/j.mcpro.2025.101472; |
| 10.6019/PXD063898; |
| Suman S, Geng L, Nevala WK, Moore R, Atherton C, Zhao X, Sung J, Guo R, Jakub JW, Kandasamy RK, McLaughlin SA, Pandey A, Markovic SN, Proteomic Analysis of Small Extracellular Vesicles From Lymphatic Affluents in Developing Premetastatic Niche in Melanoma. Mol Cell Proteomics, 25(1):101472(2025) [pubmed] |
Keyword List
Contact List
| Svetomir Markovic |
|---|
| contact affiliation | Department of oncology, Mayo Clinic, Rochester, Minnesota |
| contact email | markovic.svetomir@mayo.edu |
| lab head | |
| Shankar Suman |
|---|
| contact affiliation | Mayo Clinic |
| contact email | suman.shankar@mayo.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063898 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063898
- Label: PRIDE project
- Name: Quantitative proteomics of small extracellular vesicles from lymphatic fluid for analyzing the premetastatic niche in melanoma
