⮝ Full datasets listing
PXD063897
PXD063897 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterisation of human in vitro tumour-associated macrophage models to define translational relevance |
| Description | Tumour-associated macrophages (TAMs) are key components of the tumour microenvironment with a demonstrated ability to modulate anti-tumour T-cell responses and immunotherapy outcomes. With increasing realisation that the M1/M2 paradigm does not reflect the complexity of macrophage phenotypes in cancer patients, an urgent need has arisen to develop improved, translatable in vitro models for human TAMs. To address this gap, we have screened conditioned media from a panel of tumour cell lines for their ability to induce suppressive marker upregulation on human monocyte-derived macrophages (hMDMs), as well as active T-cell immunosuppression. We performed secretome characterization of these tumour-conditioned media (TCM) to shed light on cancer cell-derived soluble factors that may contribute to TAM polarisation. Furthermore, we characterized the proteomic and transcriptomic signatures of macrophages exposed to either TCM or primary ascites fluid from ovarian cancer patients and performed bioinformatics analysis to determine the most translationally relevant models of TAMs. In summary, our work provides mechanistic insights on tumour-macrophage crosstalk in the context of establishing suppressive TAM phenotypes and addresses the long-standing gap of defining translationally relevant human in vitro TAM models. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-03 |
| AnnouncementXML | Submission_2025-11-03_07:26:13.080.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shreya Ahuja |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl |
| Instrument | timsTOF Pro 2; timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-05-13 13:30:37 | ID requested | |
| ⏵ 1 | 2025-11-03 07:26:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Tumor conditioned medium, Tumor-associated macrophage, DIA, Immunotherapy, DatasetType:Proteomics |
Contact List
| Sonja Hess | |
|---|---|
| contact affiliation | AstraZeneca |
| contact email | sonja.hess@astrazeneca.com |
| lab head | |
| Elina Timosenko | |
| contact affiliation | AstraZeneca |
| contact email | elina.timosenko@astrazeneca.com |
| lab head | |
| Shreya Ahuja | |
| contact affiliation | AstraZeneca |
| contact email | shreya.ahuja@astrazeneca.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097870/ |
