PXD063883

PXD063883 is an original dataset announced via ProteomeXchange.

Title	Profiling CTBP2’s genome-wide interactome in murine macrophages during LPS-induced inflammation – oligomerization-dependent interactions
Description	To identify oligomerization dependent interactors of C-terminal binding protein 2 (CTBP2), we stably expressed CTBP2 wild type or a monomeric mutant (CTBP2 mono: CTBP2 C140Y, N144R, R147E, L156W) in Ctbp1-/-, Ctbp2-/- J774.1 cells and profiled their genome-wide interactome in LPS conditions after 3 h of stimulation. Differential profiling of CTBP2’s wild type and monomeric interactome shows oligomer-specific interactions with multiple repressors including KDM1A and the NuRD complex. Conversely, monomers retain the ability to interact with AP-1 and RNA polymerase II.
HostingRepository	PRIDE
AnnounceDate	2025-06-08
AnnouncementXML	Submission_2025-06-08_00:06:29.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-13 05:22:22	ID requested
⏵ 1	2025-06-08 00:06:30	announced

Dataset with its publication pending

submitter keyword: LPS, macrophage, J774.1,CTBP2, inflammation, oligomerization, mouse, ChIP-MS

Henriette Uhlenhaut
contact affiliation	Metabolic Programming, TUM School of Life Sciences, Technical University of Munich, Munich, Bavaria, Germany
contact email	henriette.uhlenhaut@tum.de
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD063883

PRIDE project URI

• PRIDE
  1. PXD063883
     1. Label: PRIDE project
     2. Name: Profiling CTBP2’s genome-wide interactome in murine macrophages during LPS-induced inflammation – oligomerization-dependent interactions