PXD063785

PXD063785 is an original dataset announced via ProteomeXchange.

Title	Acrolein as an alternative Broadly Reactive Activity-based Probe for Activity-based Protein Profiling of Cysteinome
Description	Iodoacetamide (IAM)-based probes have established the activity-based protein profiling (ABPP) paradigm, but their reliance on the SN2 mechanism limits the labeling of all cysteine residues, highlighting the need for complementary probes based on alternative reactions. Additionally, probe design must balance between minimizing probe size and simplifying workflow complexity. As a solution to the above issues, we propose acrolein (ACR), a simple α, β-unsaturated carbonyl compound, as an alternative broadly reactive ABPP probe. ACR is commercially available, requires no chemical modification, and its aldehyde group facilitates efficient enrichment. We developed a user-friendly chemoproteomic platform for ACR, enabling the identification of over 28,000 nucleophilic residues across three cell lines. ACR demonstrated its ability to detect reactivity changes in nucleophilic residues under native and denatured conditions, and to identify known targets of entacapone. Overall, ACR represents a versatile and efficient tool for studying functional nucleophilic sites, with potential applications in drug discovery.
HostingRepository	iProX
AnnounceDate	2025-05-08
AnnouncementXML	Submission_2025-05-08_20:58:01.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiahua Zhou
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 20:57:05	ID requested
⏵ 1	2025-05-08 20:58:02	announced

Dataset with its publication pending

submitter keyword: Acrolein (ACR), Activity-based protein profiling (ABPP), Activity-based probe (ABP), Chemoproteomics.

Mingliang Ye
contact affiliation	Dalian Institute of Chemical Physics
contact email	mingliang@dicp.ac.cn
lab head
Jiahua Zhou
contact affiliation	Dalian Institute of Chemical Physics
contact email	ivanhoe1849@gmail.com
dataset submitter

iProX dataset URI