PXD063779

PXD063779 is an original dataset announced via ProteomeXchange.

Title	Exploring the mode of action of a novel antileishmanial compound using thermal proteome profiling and other OMICs tools
Description	Compound 4, an antileishmanial compound identified in a GSK phenotypic-screen against Leishmania donovani (Leish-box)1 and not currently being actively researched for drug discovery, was investigated with the aim of elucidated its mode-of-action (MoA) in L. major and L. mexicana (two causative agents of cutaneous leishmaniasis). A novel pipeline approach was developed and utilised for this purpose; it included (1) characterising the mode-of-resistance of in vitro evolution-derived mutants via Illumina sequencing and cross-resistance studies against clinical antileishmanials and (2) investigating compound protein targets via thermal proteome profiling (TPP). TPP indicated differences between the two species in terms of the number and type of proteins being targeted by compound 4.
HostingRepository	PRIDE
AnnounceDate	2025-05-10
AnnouncementXML	Submission_2025-05-10_13:37:41.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Leishmania major; NCBI TaxID: 5664; scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 13:53:02	ID requested
⏵ 1	2025-05-10 13:37:42	announced

Dataset with its publication pending

submitter keyword: omics, thermal proteome profiling,Leishmania, mode of action

Matthias Trost
contact affiliation	Newcastle University, United Kingdom
contact email	matthias.trost@newcastle.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063779

PRIDE project URI

• PRIDE
  1. PXD063779
     1. Label: PRIDE project
     2. Name: Exploring the mode of action of a novel antileishmanial compound using thermal proteome profiling and other OMICs tools