PXD063761

PXD063761 is an original dataset announced via ProteomeXchange.

Title	Integrative glycomic analysis reveals the crucial role of protein glycosylation in fungal pathogenesis
Description	Protein glycosylation, a co- and post-translational modification that enhances the functional diversity of the proteome, contributes to various molecular and cellular functions by transferring different polysaccharides onto proteins. During the last decade, the role of glycosylation in plant pathogenic fungi has received significant attention, and glycoproteins are expected to play essential roles in various biological processes including pathogenicity. However, the comprehensive functional genetic analyses for protein glycosylation pathways and glycan structures of phytopathogenic fungi are still largely unknown. Here, we investigated the role of protein glycosylation in F. graminearum by identifying 74 genes putative genes involved in the protein glycosylation pathway and characterizing their functions, with a focus on glycan structures. Quantitative proteomics analysis revealed that two key players in the initial core N-glycosylation pathway, Alg3 and Alg12, regulate a wide range of glycoproteins, influencing protein functions and ultimately impacting the virulence of F. graminearum. This study elucidates the complex roles of glycosylation, highlighting the connections among genes involved in the protein glycosylation pathway, glycans, and glycoproteins in regulating the general biology and pathogenicity of Fusarium graminearum. It also would be the fungal glycoproteome study initiative.
HostingRepository	PRIDE
AnnounceDate	2025-06-27
AnnouncementXML	Submission_2025-06-27_14:36:53.201.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063761
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dae-Geun Song
SpeciesList	scientific name: Fusarium graminearum; NCBI TaxID: 5518;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 04:47:50	ID requested
⏵ 1	2025-06-27 14:36:53	announced

10.6019/PXD063761;

submitter keyword: Glycoprotein,QExactive, Fusarium

Dae-Geun Song
contact affiliation	Center for Natural Product Systems Biology, Korea Institute of Science and Technology
contact email	dsong82@kist.re.kr
lab head
Dae-Geun Song
contact affiliation	Korea Institute of Science and Technology
contact email	dsong82@kist.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063761
     1. Label: PRIDE project
     2. Name: Integrative glycomic analysis reveals the crucial role of protein glycosylation in fungal pathogenesis