PXD063754

PXD063754 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic and Metabolomic Profiling Reveals Altered Purine Metabolism in Acute Kidney Injury after On-pump Coronary Artery Bypass Graft
Description	Acute kidney injury (AKI) is a frequent complication following on-pump coronary artery bypass graft surgery (CABG). To better investigate effect of CABG on kidney function and the pathological process of AKI during the operation, the urine metabolic and protein signatures of four timepoints in 55 postoperative AKI and 104 non-AKI patients after CABG were analyzed by using high-resolution LC-MS/MS combined with pattern recognition and functional annotation. In the longitudinal urinary metabolomics study, protein catabolism pathway is prominent in AKI patients throughout the surgery and recovery process. The integration of metabolomic and proteomic data revealed that alterations in purine metabolism exhibited significant differences preoperatively between the AKI and non-AKI cohorts. A panel of two proteins (ARFIP1 and SOD2) and two metabolites (Tyrosyl-Gamma-glutamate and L-Methionine) was discovered to have a good predicting performance (area under the curve, 0.92) in the preoperative urine. Our research suggests that the panel could play a significant role in distinguishing AKIs from non-AKIs prior to surgery. We propose targeting purine metabolism pathway may help to identify potential renal protective approaches in the acute kidney injury. This study helps to reveal changes in urinary metabolomics and proteomics after CABG and may provide diagnostic clues for patients with postoperative renal injury.
HostingRepository	iProX
AnnounceDate	2025-05-08
AnnouncementXML	Submission_2025-05-07_20:40:20.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyan Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 20:39:36	ID requested
⏵ 1	2025-05-07 20:40:21	announced

Dataset with its publication pending

submitter keyword: AKI,metabolomics,proteomics

Wei Sun
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	sunwei1018@sina.com
lab head
Xiaoyan Liu
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	xiaoyanliu.good@163.com
dataset submitter

iProX dataset URI