PXD063745

PXD063745 is an original dataset announced via ProteomeXchange.

Title	Intralysosomal pathogens differentially influence the proteolytic potential of their niche
Description	Avoiding lysosomal degradation is vital to the success of intracellular pathogens. The Gram-negative bacterium C. burnetii and protozoan parasites of the Leishmania genus share the unique ability to replicate within a host phagolysosomal compartment, though the exact mechanisms utilised to withstand this hostile environment are not clearly defined. We recently reported that C. burnetii removes the lysosomal protease cathepsin B during infection of mammalian cells. Here, we aimed to determine if this virulence strategy was also employed by the intralysosomal pathogen L. mexicana. The activity of specific cathepsins was analysed, using immunoblotting and protease activity-based probes, and the changes observed during infection with C. burnetii were not observed during L. mexicana infection. Co-infection of THP-1 macrophage like cells with both pathogens resulted in a proteolytic and secretory phenotype consistent with C. burnetii infection, suggesting that C. burnetii-induced remodelling of the lysosome is not influenced by L. mexicana. The host cell proteome and secretome of L. mexicana infected cells was defined using mass spectrometry. This confirmed that, unlike C. burnetii, L. mexicana does not induce increased abundance of lysosomal proteins either intracellularly or in the extracellular milieu. Collectively, this study reveals that although C. burnetii and L. mexicana reside in a phagolysosomal intracellular niche, they employ divergent mechanisms to survive within this hostile compartment.
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-22_06:20:38.668.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauren Bird
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 16:27:13	ID requested
⏵ 1	2025-12-22 06:20:39	announced

10.1128/IAI.00270-25;

submitter keyword: Leishmania mexicana, coinfection,Coxiella burnetii, cathepsin B, cathepsin D

Prof Hayley Newton
contact affiliation	Infection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, VIC 3800, Australia
contact email	hayley.newton@monash.edu
lab head
Lauren Bird
contact affiliation	The University of Melbourne/Monash University
contact email	lauren.bird@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD063745

PRIDE project URI

• PRIDE
  1. PXD063745
     1. Label: PRIDE project
     2. Name: Intralysosomal pathogens differentially influence the proteolytic potential of their niche