PXD063732

PXD063732 is an original dataset announced via ProteomeXchange.

Title	Insects evolved a monomeric histone-fold domain in the CENP-T protein family
Description	The histone fold domain (HFD) is a conserved protein interaction module that requires stabilization through a handshake interaction with an HFD partner. All HFD proteins known to date form obligate dimers to shield the extensive hydrophobic residues along the HFD. Here, we find that the lepidopteran kinetochore protein CENP-T is soluble as a monomer. We attribute this stability to a structural rearrangement, which leads to the repositioning of the HFD helix �3. This brings a conserved two-helical extension closer to the histone fold, where it takes over the position and function of the CENP-T partner CENP-W. This change has no effect on the DNA binding ability of the lepidopteran CENP-T. Our analysis suggests that the monomeric HFD originated in the last common ancestor of insects, with a possible second independent origin in acariformes, both of which lack CENP-W. Our study highlights an unexpected structural variation in a protein module as conserved and optimized as the HFD providing a unique perspective on the evolution of protein structure and the forces driving it.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_07:58:32.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 08:33:31	ID requested
⏵ 1	2025-09-17 07:58:33	announced

Dataset with its publication pending

submitter keyword: histones, evolution, insects, structure

Ines Drinnenberg
contact affiliation	Institut Curie
contact email	ines.drinnenberg@curie.fr
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063732
     1. Label: PRIDE project
     2. Name: Insects evolved a monomeric histone-fold domain in the CENP-T protein family