PXD063728

PXD063728 is an original dataset announced via ProteomeXchange.

Title	Mitotic MAX bookmarking drives MYC-dependent hyper-transcription at TBP-bound promoters
Description	Shortly after cell division, a robust wave of hyper-transcription reactivates the genome1-3. This phenomenon is particularly pronounced in pluripotent cells4, which necessitate rapid transcriptome reactivation to maintain their undifferentiated state and prevent premature differentiation. While recent work has illuminated how specific groups of genes are reactivated4-8, the mechanisms enabling the global, efficient and accurate post-mitotic reactivation of the genome remain unknown. Here we elucidate the direct involvement of the MYC/MAX transcription factors in the post-mitotic reactivation of pluripotent mouse embryonic stem cells. While MYC undergoes extensive phosphorylation and largely dissociates from its DNA binding sites during mitosis, we report that MAX remains bound to its targets, preferentially at promoters, and facilitates early recruitment of MYC following mitosis. Through the application of MYC/MAX heterodimerization inhibitors, we demonstrate their indispensable role in sustaining hyper-transcription in ES cells, including during the critical transition from mitosis to G1 phase. Our findings uncover a novel role for MAX in mitotic bookmarking, highlighting its pivotal role in post-mitotic MYC recruitment and the re-establishment of high global transcription levels. These findings hold significant implications for medically relevant contexts, particularly when cell proliferation is of paramount importance. We anticipate that the study of mitotic bookmarking by MYC and MAX and of the effects of anti-cancer drugs targeting MYC/MAX interactions in such process will be relevant for our understanding of cancer and its potential treatments.
HostingRepository	PRIDE
AnnounceDate	2026-07-09
AnnouncementXML	Submission_2026-07-09_08:47:27.294.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monomethylated residue; half cystine; phosphorylated residue; acetylated residue; monohydroxylated residue; dimethylated residue; deamidated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 07:55:18	ID requested
⏵ 1	2026-07-09 08:47:27	announced

Dataset with its publication pending

submitter keyword: Hyper-transcription,MYC/MAX, Mitotic Bookmarking, ES cells

Pablo NAVARRO GIL
contact affiliation	UMR7338, Developmental and stem cell biology, Pasteur Institut, FRANCE
contact email	pablo.navarro-gil@pasteur.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD063728

PRIDE project URI

• PRIDE
  1. PXD063728
     1. Label: PRIDE project
     2. Name: Mitotic MAX bookmarking drives MYC-dependent hyper-transcription at TBP-bound promoters