PXD063721

PXD063721 is an original dataset announced via ProteomeXchange.

Title	Mechanistic Divergence Between SOS Response Activation and Antibiotic-Induced Plasmid Conjugation in E. coli
Description	Project description: Plasmids, frequently carried by environmental and pathogenic bacteria, play a critical role in rapid dissemination of antibiotic resistance through conjugation. The use of antibiot-ics further induces this conjugative transmission between bacteria and thereby plays a role in the rise of antibiotic resistance. This highlights the need for a fundamental understanding of the regu-lation of transmission, to limit the problem. In addition, antibiotics induce the bacterial SOS re-sponse, however, whether using SOS as target can reduce conjugation, has been unclear. In this research, we used SOS-inducing compounds, SOS-mutants, conjugation and proteomics, and showed that antibiotic induced conjugation and induction of the SOS response happens through different mechanisms. This provides a new insight, which affects future strategies to control the spread of antibiotics.
HostingRepository	PRIDE
AnnounceDate	2025-05-08
AnnouncementXML	Submission_2025-05-08_03:06:48.341.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063721
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arkadiusz Nawrocki
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 05:28:02	ID requested
⏵ 1	2025-05-08 03:06:48	announced

10.6019/PXD063721;

submitter keyword: SOS response

Conjugation

Antibiotics

E. coli

IncI1

Line Elnif Thomsen, Associate professor M.Sc. PhD
contact affiliation	University of Copenhagen Department of Veterinary and Animal Sciences Stigbøjlen 4 Frederiksberg C, Denmark
contact email	leth@sund.ku.dk
lab head
Arkadiusz Nawrocki
contact affiliation	University of Southern Denmark, Odense
contact email	arn@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063721

PRIDE project URI

• PRIDE
  1. PXD063721
     1. Label: PRIDE project
     2. Name: Mechanistic Divergence Between SOS Response Activation and Antibiotic-Induced Plasmid Conjugation in E. coli