PXD063718

PXD063718 is an original dataset announced via ProteomeXchange.

Title	Phosphite Inhibits Phytophthora cinnamomi by Downregulating Oxidoreductases and Disrupting Energy Metabolism
Description	Phytophthora root rot caused by the hemibiotrophic oomycete, Phytophthora cinnamomi is a major biotic hindrance in meeting the ever-increasing demand for avocados. In addition, the pathogen is a global menace to agriculture, horticulture and forestry. Phosphite trunk injections/foliar sprays remains the most effectual chemical management strategy followed in commercial avocado orchards to control the pathogen. Phosphite is known to counter the pathogen both directly and indirectly through fortification of host defense. However, phosphite’s direct mode of action is still not understood completely. This study identified a P. cinnamomi isolate GKB4 sensitive to phosphite (EC50 of 27.9 μg/mL) and investigated the phosphite’s direct impact on this isolate through label-free quantitative SWATH-MS. Proteomics data analysis of untreated vs phosphite-treated samples revealed that the xenobiotic affects the pathogen’s growth by targeting the oxidoreductases whose abundance is significantly reduced. Further, perturbations in the energy metabolism and membrane/transmembrane proteins and transporters, and oxidative stress contribute to growth inhibition. The current study also identified increased putrescine biosynthesis, a polyamine, that when present at non-optimal concentrations could be cytostatic/cytotoxic. The differential expression of enzymes involved in the biosynthesis of secondary metabolites and the intermediates/precursors involved in their biosynthesis is an interesting finding that needs further investigation to ascertain their role in phosphite induced stress. The pathogen’s counteraction in the form of upregulation of alternate bioenergetics pathways (amino acid catabolism and -oxidation of fatty acids), mitochondrial translation and translocation machinery, peroxisomal proteins, and antioxidants are apparently ineffective in arresting the phosphite’s growth-inhibitory effects. This research furthers our limited understanding of the direct in vitro effects of phosphite on P. cinnamomi and has identified potential candidates for molecular functional investigation.
HostingRepository	PRIDE
AnnounceDate	2025-08-01
AnnouncementXML	Submission_2025-08-01_01:29:15.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	S. Ashok Prabhu
SpeciesList	scientific name: Phytophthora cinnamomi; NCBI TaxID: 4785;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 03:42:42	ID requested
⏵ 1	2025-08-01 01:29:15	announced

10.3389/FMICB.2025.1632726;

submitter keyword: metabolism, Phytophthora cinnamomi, phosphite, oxidoreductases, membrane proteins,SWATH-MS, antioxidants, mitochondrial translation

Noëlani van den Berg
contact affiliation	Hans Merensky Chair in Avocado Research, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0028, South Africa
contact email	noelani.vdberg@fabi.up.ac.za
lab head
S. Ashok Prabhu
contact affiliation	University of Pretoria
contact email	ashokprabhus007@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063718

PRIDE project URI

• PRIDE
  1. PXD063718
     1. Label: PRIDE project
     2. Name: Phosphite Inhibits Phytophthora cinnamomi by Downregulating Oxidoreductases and Disrupting Energy Metabolism