PXD063703

PXD063703 is an original dataset announced via ProteomeXchange.

Title	LipoID: a Photochemical Proteomic Approach to in situ Profile Lipid Droplets Interactions and Identify Inter-Organelle Regulators
Description	Lipid droplets (LDs) dynamically interact with other organelles, such as mitochondria, in surveillance of cellular metabolic homeostasis. The transient nature of LDs, however, poses technical challenges to snapshot molecular information underlying these interactions. Herein, we develop a small molecule based photocatalytic protein proximity labeling method (namely LipoID) to in situ label, capture and profile LDs’ interacting proteome. This method is enabled by a set of LDs-targeting probes designed to catalyze protein modifications nearby LDs using nucleophilic substrates. Profiled by LC-MS/MS, LipoID identifies tethered inter-organellar interactions, particularly with mitochondria, besides reliable capture of validated LDs’ biomarkers (e.g. PLINs). Coupled with comparative proteomics, LipoID discovers mitochondrial VDAC3 channel and its inhibitor that regulate the LDs-mitochondria distance. Such inter-organellar regulation was driven by cellular ATP transported through the VDAC3 channel. Further metabolomics analysis revealed remodeled lipid metabolism orchestrated with LDs-mitochondria interaction. Together, LipoID profiles LDs’ interactome and reveals inter-organellar regulation.
HostingRepository	iProX
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_22:50:30.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Zhao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-06 22:49:59	ID requested
⏵ 1	2025-05-06 22:50:30	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, Lipid droplets, a Photochemical Proteomic Approach

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	lihuazhang@dicp.ac.cn
lab head
Nan Zhao
contact affiliation	Dalian Institute of Chemical Physics
contact email	zhaonan@dicp.ac.cn
dataset submitter

iProX dataset URI