PXD063690
PXD063690 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Novel Strategy to Rapidly Profile and Quantify High-Risk Host Cell Proteins using Integrated DDA-PRM-dMRM Mode |
Description | We developed a novel strategy using integrated DDA-PRM-dMRM to comprehensively profile and quantify high-risk HCPs in CHO cell-derived biotherapeutics. Firstly, we performed deep proteomic analysis of CHO cells using DDA-PASEF mode, constructing a high-quality spectral library covering all potential HCPs. Based on the constructed library, we validated the target list using PRM-PASEF and MRM modes. |
HostingRepository | iProX |
AnnounceDate | 2025-05-03 |
AnnouncementXML | Submission_2025-05-06_20:03:35.445.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xuan Xu |
SpeciesList | scientific name: Cricetulus griseus; NCBI TaxID: 10029; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro; 6490 Triple Quadrupole LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-05-06 20:02:48 | ID requested | |
⏵ 1 | 2025-05-06 20:03:35 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Host cell proteins |
LC-MS/MS |
Biotherapeutic impurities |
Chinese hamster ovary cell |
Proteomics |
Contact List
Jinlan Zhang | |
---|---|
contact affiliation | Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Materia Medica |
contact email | zhjl@imm.ac.cn |
lab head | |
Xuan Xu | |
contact affiliation | Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Materia Medica |
contact email | xuxuan@imm.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |