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PXD063687

PXD063687 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMild Tissue Extraction for Mass Spectrometry-based Proteomic Discovery of Biomarkers
DescriptionClinical blood biomarkers provide important diagnostic information and are generally secreted, cytoplasmic, membrane and other soluble proteins. However, in the mass spectrometry-based proteomics-based discovery phase, the whole tissue is often lysed and analyzed, increasing protein complexity and thus limiting the discovery of those soluble biomarkers. Here we use a mild extraction method that utilizes low salt buffer and 1% Triton X-100 to extract proteins from the tissue sample followed by the proteome analysis. This mild condition extracts membrane and cytoplasmic proteins efficiently while spares nuclear proteins largely from the mouse brain tissue as demonstrated by Western blot. The subsequent proteomic analysis shows significant enrichment of secreted and cytoplasmic proteins in the low salt and Triton extracts. Expanding application of this method to other 16 mouse tissue samples has yielded similar results. Further in-depth proteomic analysis of an Alzheimer mouse brain tissue mild extract has identified more than 12,000 proteins with significant enrichment in the soluble proteins and decent depth of serum proteins. Overall, this mild tissue extraction demonstrates to be an efficient approach for proteomic discovery of circulating biomarkers.
HostingRepositoryiProX
AnnounceDate2025-05-02
AnnouncementXMLSubmission_2025-05-06_19:56:48.281.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBing Bai
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-06 19:56:17ID requested
12025-05-06 19:56:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Tissue, mild extraction, Low salt, Triton, secreted proteins, cytoplasmic proteins, membrane proteins, proteomics, Biomarker Discovery
Contact List
Bing Bai
contact affiliationNanjing University
contact emailbing.bai@nju.edu.cn
lab head
Bing Bai
contact affiliationNanjing University
contact emailbing.bai@nju.edu.cn
dataset submitter
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