PXD063684

PXD063684 is an original dataset announced via ProteomeXchange.

Title	TMT-based proteomics analysis identifies RPLP1 as a key protein target in ursolic acid inhibition of colorectal cancer
Description	Purpose: Ursolic Acid (UA), a triterpenoid extracted from Hedyotis Diffusa Willd. (HDW), is known for its anti-inflammatory, antioxidant, and antitumor effects. Nevertheless, the mechanisms underlying UA's anti-colorectal cancer (CRC) effects remain insufficiently understood. This study is aim to identify the key target proteins of UA and investigated their functions in CRC development. Methods: The cytotoxicity of three active components of HDW (UA, oleanolic acid (OA), and quercetin) on CRC cells was evaluated using the CCK-8 assay. Tandem mass tag (TMT)-based proteomics was employed to detect differentially expressed proteins (DEPs) in CRC cells after UA treatment. Bioinformatics analysis and high-content screening were used to identify UA’s key protein targets. The expression and role of RPLP1 in CRC cells were investigated, including the effects of RPLP1 knockdown and its combination with UA treatment on cell proliferation, migration, invasion, and apoptosis. Results: UA demonstrated superior inhibitory effects on CRC cells compared to OA and quercetin, highlighting it as a principal active ingredient of HDW. TMT-based proteomic analysis identified revealed 438 upregulated and 366 downregulated proteins after UA intervention. Among these, RPLP1 was identified as a critical target. UA inhibited RPLP1 expression in CRC cells, resulting in decreased cell proliferation, migration, and invasion. Furthermore, combination of UA treatment and RPLP1 knockdown exhibited synergistic effects in inhibiting CRC cell growth and migration, as well as promoting apoptosis. Conclusions: UA, a bioactive triterpenoid of HDW, inhibits CRC development by targeting and suppressing RPLP1 expression. These findings provide novel insights into the therapeutic of UA for CRC and highlight RPLP1 as a promising target for intervention.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:23:45.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Limin Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-06 19:16:54	ID requested
⏵ 1	2026-02-08 16:23:46	announced

10.1007/s12672-025-03486-z;

Zhu LM, Shi HX, Xu ZY, Deng HB, TMT-based proteomics analysis identifies RPLP1 as a key protein target in ursolic acid Inhibition of colorectal cancer. Discov Oncol, 16(1):1665(2025) [pubmed]

submitter keyword: Colorectal cancer

Hedyotis Diffusa Willd

Ursolic acid

RPLP1

Tandem mass tags

Li-Min Zhu
contact affiliation	Department of Oncology, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, No. 725 Wanping South Road, Xuhui District, Shanghai, 200032, China.
contact email	zhu.limin24@outlook.com
lab head
Limin Zhu
contact affiliation	LongHua Hospital, Shanghai University of Traditional Chinese Medicine
contact email	zhu.limin24@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063684
     1. Label: PRIDE project
     2. Name: TMT-based proteomics analysis identifies RPLP1 as a key protein target in ursolic acid inhibition of colorectal cancer