PXD063683

PXD063683 is an original dataset announced via ProteomeXchange.

Title	Lipid-Coated Nanoparticles Enable Enhanced Delivery of Bacterial Virulence Factors as a Potent Toxoid Vaccine Platform against Bacterial Infections
Description	Antivirulence vaccination represents a promising strategy for infection prevention, but achieving both safety and efficacy in toxoid vaccine preparation remains a challenge. Cell membrane-based nanotoxoids offer a safe delivery platform for bacterial virulence factors in antivirulence vaccination, but limited absorption capacity hampers their efficacy. Here, we develop a lipid-based toxoid vaccine platform, PSV-CNP, comprising a CpG-loaded polymeric core coated with phosphatidylcholine/sphingomyelin (PS) liposomes enriched with bacterial virulence factors. By enhancing virulence factor absorption, PSV-CNP elicits robust humoral immunity. In mice, it provides long-lasting protection against methicillin-resistant Staphylococcus aureus (MRSA) and clinically isolated S. aureus (CI-SA), even under immunosuppression. In Bama pigs, PSV-CNP induces strong immune responses and prevents MRSA and CI-SA invasion. Furthermore, PS-liposomes efficiently absorb virulence factors from Pseudomonas aeruginosa (PA), conferring protection against PA infections. This study establishes PS-coated nanoparticles as a broadly applicable, safe, and effective antivirulence toxoid vaccine platform.
HostingRepository	PRIDE
AnnounceDate	2025-05-09
AnnouncementXML	Submission_2025-05-08_19:04:43.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deli Zhuge
SpeciesList	scientific name: Staphylococcus aureus (strain USA300); NCBI TaxID: 367830; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker TSF format

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-06 19:16:07	ID requested
⏵ 1	2025-05-08 19:04:44	announced

Dataset with its publication pending

submitter keyword: pore-forming toxin, antivirulence,toxoid vaccine, bacterial infection, liposome

Deli Zhuge
contact affiliation	Department of Pharmaceutics, School of Pharmaceutical Sciences of Wenzhou Medical University
contact email	zhugedeli@wmu.edu.cn
lab head
Deli Zhuge
contact affiliation	Wenzhou Medical University
contact email	zhugedeli@wmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063683
     1. Label: PRIDE project
     2. Name: Lipid-Coated Nanoparticles Enable Enhanced Delivery of Bacterial Virulence Factors as a Potent Toxoid Vaccine Platform against Bacterial Infections