PXD063671

PXD063671 is an original dataset announced via ProteomeXchange.

Title	Glycomics and Glycoproteomics Reveal Distinct Oligomannose Carriers Across Bladder Cancer Stages
Description	Aberrant glycosylation is a hallmark of cancer, offering opportunities to enhance clinical decision-making and enable precise targeting of cancer cells. Nevertheless, alterations in the bladder urothelial carcinoma (BLCA) N-glycome remain poorly characterized. Here, we used in situ N-deglycosylation and mass spectrometry, revealing a marked enrichment of oligomannose-type N-glycans in non-invasive Ta tumors, which diminished with disease progression. Complementary analysis of TCGA transcriptom-ic data revealed downregulation of key mannosidases in BLCA, suggesting a mechanistic basis for oligomannose accumulation, though this requires further validation. Then, targeted glycoproteomic profiling identified potential stage-specific carriers of oligomannoses. Functional annotation revealed stage-dependent glycoprotein activi-ties, ranging from metabolic regulation in Ta tumors to oxidative stress adaptation in muscle-invasive disease, highlighting glycosylation’s contribution to tumor progres-sion. Furthermore, myeloperoxidase (MPO) was enriched in more aggressive stages. Spatial validation confirmed MPO overexpression in tumor-infiltrating immune cells and its correlation with oligomannose content. Importantly, high MPO expression combined with low mannosidase levels was linked to poor survival, suggesting clinical relevance. This study suggests a dynamic, stage-specific N-glycome in BLCA and iden-tifies oligomannose-bearing glycoproteins as potential biomarkers and therapeutic targets, advancing the framework for glycan-based precision oncology.
HostingRepository	PRIDE
AnnounceDate	2025-05-20
AnnouncementXML	Submission_2025-05-20_05:14:24.343.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Relvas-Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-06 10:59:39	ID requested
⏵ 1	2025-05-20 05:14:24	announced

10.3390/IJMS26104891;

submitter keyword: bladder cancer

N-glycomics

N-glycoproteins

José Alexandre Ferreira
contact affiliation	Research Center of IPO Porto (CI-IPOP)/CI-IPOP@RISE (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC)
contact email	jose.a.ferreira@ipoporto.min-saude.pt
lab head
Marta Relvas-Santos
contact affiliation	Research Center of Portuguese Oncology Institute of Porto
contact email	marta.relvas.santos@ipoporto.min-saude.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063671
     1. Label: PRIDE project
     2. Name: Glycomics and Glycoproteomics Reveal Distinct Oligomannose Carriers Across Bladder Cancer Stages