PXD063615

PXD063615 is an original dataset announced via ProteomeXchange.

Title	A Protein Disulfide Isomerase Coordinates Redox Homeostasis and ER Calcium Regulation for Optimal Lytic Cycle Progression in Toxoplasma gondii. DVSF anti-TgPDIA3 LC-MS/MS
Description	The endoplasmic reticulum (ER) maintains an oxidative environment that promotes disulfide bond formation, a critical process for proper protein folding. Protein disulfide isomerases (PDIs) are ER resident enzymes that facilitate the formation, breakage, and rearrangement of disulfide bonds between cysteine residues, thereby stabilizing protein structures. Although PDIs are functionally diverse, they all contain at least 1 thioredoxin-like domain and mediate disulfide exchange through their conserved CXXC motifs. The Apicomplexan parasite, Toxoplasma gondii, infects approximately one third of the world population, posing a significant risk to immunosuppressed individuals and unborn fetuses. Its fast-replicating tachyzoite form engages in a lytic cycle, causing host tissue damage and contributing to pathogenesis. While approximately 26 PDIs are predicted to be present in T. gondii, their specific roles remain largely unexplored. In this study, we investigate TgPDIA3, a T. gondii PDI localized to the ER, along with several of its interacting protein substrates. We explore its role in ER redox activity and calcium sequestration and assess how these functions contribute to the parasite’s lytic cycle
HostingRepository	PRIDE
AnnounceDate	2026-03-31
AnnouncementXML	Submission_2026-03-31_13:12:23.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katherine Moen
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: NEWT:507601;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-05 12:23:55	ID requested
⏵ 1	2026-03-31 13:12:23	announced

Dataset with its publication pending

submitter keyword: anti-TgPDIA3,DVSF, LC-MS/MS

Silvia Moreno
contact affiliation	Center for Tropical and Ememrging Global Diseases and Department of Cellular Biology, Moreno lab, University of Georgia, United States of America
contact email	smoreno@uga.edu
lab head
Katherine Moen
contact affiliation	University of Georgia
contact email	katherine.moen26@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063615
     1. Label: PRIDE project
     2. Name: A Protein Disulfide Isomerase Coordinates Redox Homeostasis and ER Calcium Regulation for Optimal Lytic Cycle Progression in Toxoplasma gondii. DVSF anti-TgPDIA3 LC-MS/MS