PXD063609

PXD063609 is an original dataset announced via ProteomeXchange.

Title	APEX2-based mapping of the host-Shigella interface
Description	To discover novel factors that modulate S. flexneri infection, we developed a proteomic-based approach based on the biotin ligation enzyme APEX2. To target the host-Shigella interface, we coated bacteria in vitro prior to infection using anti-GFP nanobody-expressing shigella and recombinant GFP-APEX2. Upon infection in HeLa cells, biotinylated proteins from both host and pathogen were enriched by streptavidin pulldown and identified by mass spectrometry. Here, we analyzed two biological groups where cells were infected with either wild-type Shigella flexneri (S. flexneri) or delta-mixE Shigella flexneri mutant. In each group, four petri dishes were infected to generate four replicates.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_03:41:59.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabien Thery
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Shigella flexneri; NCBI TaxID: NEWT:623; scientific name: Shigella; NCBI TaxID: NCBITaxon:620;
ModificationList	biotinylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-05 09:05:18	ID requested
⏵ 1	2026-08-17 03:42:00	announced

Dataset with its publication pending

submitter keyword: Shigella, APEX2,human, biotin

Prof. Dr. Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium. Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
contact email	francis.impens@vib-ugent.be
lab head
Fabien Thery
contact affiliation	VIB-UGent
contact email	fabien.thery91@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD063609

PRIDE project URI

• PRIDE
  1. PXD063609
     1. Label: PRIDE project
     2. Name: APEX2-based mapping of the host-Shigella interface