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PXD063589

PXD063589 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic tracking extracellular vesicle RNA interactors through orthogonal labelings in recipient immune cells
DescriptionExtracellular vesicles (EVs) mediate cell-cell communication by transferring RNAs that modulates recipient cell function. However, the RNA-protein interactome in EV-recipient cell communication remains poorly understood due to technical limitations. We developed a novel EV RNA-protein interaction profiling by targeted crosslinking and SILAC (EV-RIP-Tag) strategy to systematically capture EV RNA-protein interactions in recipient cells. This approach enabled the first large-scale profiling of EV RNA-binding proteins (RBPs) in recipient cells. Our dynamic study of tumor-derived EVs in Jurkat T cells provided new insights into the temporal process of EV RNA uptake. Additionally, profiling EV RBPs from wild-type and IDH1-mutant ICC cell lines revealed significant changes in the RBP landscape within CD8+ T cells. These alterations were reversed upon treatment with the IDH1 inhibitor AG120, highlighting a potential mechanism for IDH1 mutation-mediated immune suppression. Our findings offer novel insights into EV-mediated immune modulation and present a powerful tool for studying the molecular mechanisms of cancer-associated immunosuppression.
HostingRepositoryjPOST
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_12:59:38.965.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZheng Zhang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; 6x(13)C labeled L-lysine; 6x(13)C labeled L-arginine; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-04 12:02:41ID requested
12025-05-07 12:59:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: extracellular vesicles, RNA binding proteins, immunosuppression, EV-recipient cell interactions
Contact List
W. Andy. Tao
lab head
Zheng Zhang
contact affiliationPurdue University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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