PXD063589

PXD063589 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic tracking extracellular vesicle RNA interactors through orthogonal labelings in recipient immune cells
Description	Extracellular vesicles (EVs) mediate cell-cell communication by transferring RNAs that modulates recipient cell function. However, the RNA-protein interactome in EV-recipient cell communication remains poorly understood due to technical limitations. We developed a novel EV RNA-protein interaction profiling by targeted crosslinking and SILAC (EV-RIP-Tag) strategy to systematically capture EV RNA-protein interactions in recipient cells. This approach enabled the first large-scale profiling of EV RNA-binding proteins (RBPs) in recipient cells. Our dynamic study of tumor-derived EVs in Jurkat T cells provided new insights into the temporal process of EV RNA uptake. Additionally, profiling EV RBPs from wild-type and IDH1-mutant ICC cell lines revealed significant changes in the RBP landscape within CD8+ T cells. These alterations were reversed upon treatment with the IDH1 inhibitor AG120, highlighting a potential mechanism for IDH1 mutation-mediated immune suppression. Our findings offer novel insights into EV-mediated immune modulation and present a powerful tool for studying the molecular mechanisms of cancer-associated immunosuppression.
HostingRepository	jPOST
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_12:59:38.965.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheng Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; 6x(13)C labeled L-lysine; 6x(13)C labeled L-arginine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-04 12:02:41	ID requested
⏵ 1	2025-05-07 12:59:39	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: extracellular vesicles, RNA binding proteins, immunosuppression, EV-recipient cell interactions

submitter keyword: extracellular vesicles, RNA binding proteins, immunosuppression, EV-recipient cell interactions

Contact List

W. Andy. Tao
lab head
Zheng Zhang
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003710/