PXD063587

PXD063587 is an original dataset announced via ProteomeXchange.

Title	Association of heme bound HRI with S-DELE1 promotes structural re-arrangements within the HRI kinase domain
Description	This dataset assesses structural changes occurring in human, heme-bound HRI upon association with S-DELE1. To this end, human HRI and S-DELE1 were expressed EIF2alpha phosphorylation-resistent cells supplemented with hemin. HRI was affinity purified, crosslinked with DSS, and structural changes in HRI were assessed by MS. The data set identifies S-DELE1 induced structural changes in the C-terminal region and the kinase insert of HRI, as well as domains of HRI that are in structural proximity to S-DELE1.
HostingRepository	PRIDE
AnnounceDate	2026-05-07
AnnouncementXML	Submission_2026-05-07_03:05:16.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-04 09:42:11	ID requested
⏵ 1	2026-05-07 03:05:17	announced

Dataset with its publication pending

submitter keyword: HRI, heme,DELE1, XL-MS

Lucas Jae
contact affiliation	Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
contact email	Jae@genzentrum.lmu.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD063587

PRIDE project URI

• PRIDE
  1. PXD063587
     1. Label: PRIDE project
     2. Name: Association of heme bound HRI with S-DELE1 promotes structural re-arrangements within the HRI kinase domain