PXD063564

PXD063564 is an original dataset announced via ProteomeXchange.

Title	TMT-Based Quantitative Proteomics of Cerebrospinal Fluid Reveals Molecular Signatures of Lymphatic Drainage Impairment in Young Rats
Description	The glymphatic system facilitates the clearance of interstitial solutes and waste products from the brain parenchyma, channeling them into the meningeal lymphatic vessels for subsequent drainage into the deep cervical lymph nodes (dcLNs). However, the extent to which this lymphatic outflow via dcLNs is essential for maintaining cerebral homeostasis remains insufficiently understood. To address this, we performed high-resolution proteomic profiling of cerebrospinal fluid (CSF) from 3-month-old rats using Tandem Mass Tag (TMT)–based quantitative mass spectrometry. This approach enabled the sensitive detection of proteomic perturbations in the CSF resulting from impaired lymphatic drainage. A total of six CSF samples from 3-month-old rats (n=6) were analyzed. TMT 10-plex–based quantitative proteomics revealed 4,089 proteins, among which 357 exhibited statistically significant differential expression (p < 0.05). These proteomic alterations indicated early signs of cellular stress and low-grade inflammation. Pathway enrichment analysis further revealed upregulation of molecular cascades associated with neurodegenerative processes, even in this relatively young cohort, following lymphatic disruption.
HostingRepository	PRIDE
AnnounceDate	2025-07-04
AnnouncementXML	Submission_2025-07-04_07:22:49.076.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063564
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ZOHAIB KHAN
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-02 09:58:47	ID requested
⏵ 1	2025-07-04 07:22:49	announced

10.6019/PXD063564;

submitter keyword: dc-LN Cauterization,CSF

Laura Santambrogio
contact affiliation	Englander Institute of Precision Medicine Professor Radiation Oncology Professor Physiology and Biophysics Weill Cornell Medicine 1300 York Avenue Room E215 New York, NY 10065
contact email	las4011@med.cornell.edu
lab head
ZOHAIB KHAN
contact affiliation	WEILL CORNELL MEDICINE
contact email	znk4001@med.cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063564
     1. Label: PRIDE project
     2. Name: TMT-Based Quantitative Proteomics of Cerebrospinal Fluid Reveals Molecular Signatures of Lymphatic Drainage Impairment in Young Rats