PXD063561

PXD063561 is an original dataset announced via ProteomeXchange.

Title	The C-terminal end of engineered albumin variants and fusion proteins after exposure to carboxypeptidase A
Description	Albumin has a long plasma half-life due to engagement of the neonatal Fc receptor (FcRn), which prevents intracellular degradation. However, its C-terminal end can be cleaved by carboxypeptidase A, and removal of the last leucine residue (L585) weakens receptor binding, reducing its half-life from 20 days to 3.5 days in humans. This biology is important to consider when designing human albumin-fused biologics. Here, we explore engineering strategies to secure favorable FcRn binding and pharmacokinetic properties, and we use LC-MS/MS to analyze the amino acid sequence of the C-terminal end of engineered albumin variants and albumin fusion proteins, following incubation with or without carboxypeptidase A.
HostingRepository	PRIDE
AnnounceDate	2025-05-19
AnnouncementXML	Submission_2025-05-19_00:19:18.267.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ingrid Maria Erika Ekman Stensland
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-02 07:58:09	ID requested
⏵ 1	2025-05-19 00:19:18	announced

Dataset with its publication pending

submitter keyword: albumin engineering, Carboxypeptidase A,The neonatal Fc receptor (FcRn), albumin-fusion proteins

Tuula A. Nyman
contact affiliation	The Proteomics Core Facility (PCF) at Oslo University Hospital
contact email	t.a.nyman@medisin.uio.no
lab head
Ingrid Maria Erika Ekman Stensland
contact affiliation	Oslo University hospital
contact email	maria.stensland@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063561

PRIDE project URI

• PRIDE
  1. PXD063561
     1. Label: PRIDE project
     2. Name: The C-terminal end of engineered albumin variants and fusion proteins after exposure to carboxypeptidase A