PXD063552

PXD063552 is an original dataset announced via ProteomeXchange.

Title	Altered Metabolomics and Inflammatory Transcriptomics in Human Bone Marrow Adipocytes After Acute High Calorie Diet and Acute Fasting
Description	Investigated bone marrow metabolism in response to acute overnutrition (high calorie diet) and calorie deprivation (fasting). Bone marrow serum and peripheral serum were collected before and after each dietary intervention. Using proteomics analyses of the sera, we characterized nutritional influences on the local and systemic environments. In brief, proteomic analysis of the bone marrow serum after high calorie diet showed an upregulation of proteins related to an inflammatory/complement pathway, while bone marrow serum after fasting showed a significant downregulation of inflammatory/complement pathway proteins. In contrast, the peripheral serum showed signs of an increased inflammatory response that may be due to a rapid increase in body weight and fat mass seen in participants after 10 days of a high calorie diet. After fasting, peripheral serum did show a strong inflammatory response, but there was evidence of increased lipid biosynthesis. Overall, our results demonstrate key differences in inflammatory response and lipid metabolism between high calorie diet and fasting, despite a nearly identical bone marrow adipose phenotype.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_17:32:59.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos Gartner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-02 03:40:11	ID requested
⏵ 1	2025-07-13 17:33:00	announced

Costa SN, Pach, ó, n-Pe, ñ, a G, Dragon J, Tighe S, Vary C, Fazeli PK, Bredella MA, Rosen CJ, Altered metabolomics and inflammatory transcriptomics in human bone marrow adipocytes after acute high calorie diet and acute fasting. Front Endocrinol (Lausanne), 16():1591280(2025) [pubmed]

10.3389/fendo.2025.1591280;

ProteomeXchange project tag: Cardiovascular (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Food and Nutrition (B/D-HPP), Human Proteome Project

submitter keyword: human

acute high calorie diet

acute fasting

bone marrow serum

peripheral blood serum

proteomics

mass spectrometry

Calvin Vary
contact affiliation	MaineHealth Institute for Research Proteomics and Lipidomics Core Facility
contact email	Calvin.Vary@mainehealth.org
lab head
Carlos Gartner
contact affiliation	MaineHealth Institute for Research
contact email	gusti.gartner@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063552

PRIDE project URI

• PRIDE
  1. PXD063552
     1. Label: PRIDE project
     2. Name: Altered Metabolomics and Inflammatory Transcriptomics in Human Bone Marrow Adipocytes After Acute High Calorie Diet and Acute Fasting