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PXD063535

PXD063535 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends
DescriptionCDK7 regulates RNA polymerase II (RNAPII) initiation, elongation, and termination through incompletely understood mechanisms. Because contaminating kinases precluded CDK7 analysis with nuclear extracts, we completed biochemical assays with purified factors. Reconstitution of RNAPII transcription initiation showed CDK7 inhibition slowed and/or paused RNAPII promoterproximal transcription, which reduced re-initiation. These CDK7-regulatory functions were suppressing RNAPII activity at promoters, consistent with reduced initiation and/or re-initiation. Moreover, widespread 3'-end readthrough transcription was observed in CDK7-inhibited cells; mechanistically, this occurred through rapid nuclear depletion of RNAPII elongation and termination factors, including high-confidence CDK7 targets. Collectively, these results define how CDK7 governs RNAPII function at gene 5'-ends and 3'-ends, and reveal that nuclear abundance of elongation and termination factors is kinase-dependent. Because 3'-readthrough transcription is commonly induced during stress, our results further suggest regulated suppression of CDK7 activity may enable this RNAPII transcriptional response.
HostingRepositoryPRIDE
AnnounceDate2025-05-08
AnnouncementXMLSubmission_2025-05-08_14:36:33.119.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD063535
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Ebmeier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-01 08:51:34ID requested
12025-05-08 14:36:33announced
Publication List
10.6019/PXD063535;
10.1101/2025.01.08.632016;
Keyword List
submitter keyword: CDK7 regulates RNAPII
Contact List
Dylan J Taatjes
contact affiliationProfessor
contact emaildylan.taatjes@colorado.edu
lab head
Christopher Ebmeier
contact affiliationUniversity of Colorado Boulder
contact emailchristopher.ebmeier@colorado.edu
dataset submitter
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