PXD063532

PXD063532 is an original dataset announced via ProteomeXchange.

Title	SH3P2-mediated Autophagosomal Targeting of the CCZ1-MON1-RABG3e Module Regulates Autophagosome-Vacuole Fusion in Arabidopsis
Description	Autophagy is a highly conserved pathway responsible for the bulk degradation of cytoplasmic material through the formation of a double-membrane structure known as the autophagosome. However, the precise mechanisms governing the transport of autophagosomes to the vacuole for degradation in plants remain largely elusive. There exists an ongoing debate about whether RAB7, a key regulatory protein, is involved in the plant autophagy pathway. In this study, we demonstrate that upon autophagy induction by BTH treatment, RABG3e, a member of the RAB7 family, exhibits a partial localization with late-stage autophagosomes in Arabidopsis root cells, and its dysfunction leads to the accumulation of enlarged multilayered autophagosomes and a significant reduction in autophagic flux. We also showed that RABG3e is recruited to autophagosomes by its GEF (guanine nucleotide exchange factor) complex, MON1-CCZ1, which is targeted through the interaction between CCZ1 and SH3P2 (SH3 domain-containing protein 2), a plant-specific autophagy regulator. Subsequently, RABG3e recruits the HOPS (homotypic fusion and vacuole protein sorting) complex, which facilitates the recruitment of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) proteins, including SYP21, VTI12, SYP51, and VAMP711, that are essential for the fusion process. Arabidopsis mutants with dysfunction in the autophagosome-vacuole fusion process exhibit accelerated senescence and increased sensitivity to nitrogen starvation. Collectively, our findings provide new insights into the regulation of autophagosome-vacuole fusion in Arabidopsis, highlighting the essential roles of SH3P2-dependent targeting of the CCZ1-MON1-RABG3e module to late-stage autophagosomes as well as the RAB7 effector HOPS and the unique SNARE complex.
HostingRepository	PRIDE
AnnounceDate	2025-11-26
AnnouncementXML	Submission_2025-11-26_03:18:54.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaohui Zheng
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-01 06:13:28	ID requested
⏵ 1	2025-11-26 03:18:55	announced

10.1080/15548627.2025.2593138;

submitter keyword: Arabidopsis

Yong Cui
contact affiliation	State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen 361102, China
contact email	cuiyong@xmu.edu.cn
lab head
Xiaohui Zheng
contact affiliation	21620220157051@stu.xmu.edu.cn
contact email	21620220157051@stu.xmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD063532

PRIDE project URI

• PRIDE
  1. PXD063532
     1. Label: PRIDE project
     2. Name: SH3P2-mediated Autophagosomal Targeting of the CCZ1-MON1-RABG3e Module Regulates Autophagosome-Vacuole Fusion in Arabidopsis