PXD063514

PXD063514 is an original dataset announced via ProteomeXchange.

Title	Pex3 promotes formation of peroxisome-peroxisome and peroxisome-lipid droplet contact sites
Description	Peroxisomes are ubiquitous organelles that mediate central metabolic functions, such as fatty acid β-oxidation, as well as diverse tissue- and organism-specific processes. Membrane contact sites, regions of close apposition with other organelles for direct communication, are central to several aspects of their life cycle. Pex3 is a conserved multifunctional peroxisomal transmembrane protein that is involved in the insertion of peroxisomal membrane proteins, in pexophagy, and in the formation of membrane contact sites. Here, we show that high Pex3 levels in Saccharomyces cerevisiae induce the formation of peroxisome clusters surrounded by lipid droplets, mediated by peroxisome-peroxisome and peroxisome-lipid droplet contact sites. This clustering occurs independently of Pex3 partners in other processes Pex19, Inp1, and Atg36. The cytosolic domain of Pex3 binds peroxisomes, suggesting a direct role in homotypic contact site formation. Lipid droplet-peroxisome contact sites require the lipid droplet-localized triacylglycerol lipase Tgl4, which is enriched at this interface along with other lipases. Pex3 overexpression in Drosophila melanogaster similarly alters peroxisome and lipid droplet morphology and promotes contact site formation. Together, our results offer novel molecular insights into homotypic peroxisome contact sites and peroxisome-lipid droplet contact sites across species.
HostingRepository	PRIDE
AnnounceDate	2025-05-22
AnnouncementXML	Submission_2025-05-22_10:12:03.129.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063514
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ayelén González Montoro
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 14:12:39	ID requested
⏵ 1	2025-05-22 10:12:03	announced

Dataset with its publication pending

submitter keyword: Yeast TurboID Pex3 contact sites peroxisomes lipid droplets

Cellular Communication Laboratory - Gonzalez Montoro
contact affiliation	Cellular Communication Laboratory, Department of Biology/Chemistry, Osnabrück University Barbarastrasse 13, 49076, Osnabrück, Germany
contact email	ayelen.gonzalez.montoro@uos.de
lab head
Ayelén González Montoro
contact affiliation	Osnabrück University
contact email	ayelen.gonzalez.montoro@uos.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063514

PRIDE project URI

• PRIDE
  1. PXD063514
     1. Label: PRIDE project
     2. Name: Pex3 promotes formation of peroxisome-peroxisome and peroxisome-lipid droplet contact sites