PXD063507

PXD063507 is an original dataset announced via ProteomeXchange.

Title	RBR1-E2F/DP transcriptional regulation modulated by the F-box protein FBL17
Description	In all eukaryotes, F-box proteins of SCF E3 ligase complexes have been well-documented to control the abundance of numerous critical regulatory proteins. In Arabidopsis, one of them, F-BOX-LIKE17 (FBL17), stands out for playing a key role in DNA replication, DNA damage and more recently for the control of cell size. FBL17 null mutants exhibit severe cellular defects leading to lethality, presumably due to impaired degradation of cyclin-dependent kinase inhibitor proteins. However, the molecular mechanisms by how FBL17 operate remain poorly understood. Here we show that FBL17 interacts with different components of the RETINOBLASTOMA-RELATED1/E2F module. In particular, FBL17 directly targets the two transcription factors E2Fa and E2Fb and is involved in their protein turnover. However, it is the transcriptional repressor E2Fc, which is not a substrate of FBL17, that causes fbl17 mutant lethality. Hence, the e2fc mutation broadly suppresses cell elongation and cell proliferation defects observed in fbl17. Moreover, in the fbl17 e2fb double mutant the strong overexpression of DNA damage genes is significantly attenuated through RBR1 recruitment to its target genes. Altogether, our results highlight a key role for FBL17 in modulating the transcriptional control of E2F-target genes ensuring precise control of cell cycle progression and avoiding uncontrolled DNA damage response.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:33:13.957.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 08:38:29	ID requested
⏵ 1	2026-02-22 16:33:14	announced

Espanet J, He X, Pan T, Gentric N, Potuschak T, Desvoyes B, Hammann P, Chicher J, Brik R, Lechner E, Latrasse D, Pettk, ó, -Szandtner A, Gutierrez C, Raynaud C, Magyar Z, Benhamed M, Yan S, Noir S, Genschik P, Tuning of the RBR1-E2F/DP transcriptional module by the F-box protein FBL17. Sci Adv, 12(8):eadz2439(2026) [pubmed]

10.1126/sciadv.adz2439;

submitter keyword: Cell cycle, E2F/RBR, ubiquitin, plant, F-box

Pascal Genschik
contact affiliation	Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084 Strasbourg, France
contact email	pascal.genschik@ibmp-cnrs.unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507

PRIDE project URI

• PRIDE
  1. PXD063507
     1. Label: PRIDE project
     2. Name: RBR1-E2F/DP transcriptional regulation modulated by the F-box protein FBL17