PXD063493

PXD063493 is an original dataset announced via ProteomeXchange.

Title	Proteomic Characterization of Naiavirus
Description	Giant viruses infecting amoebae are notable for their substantial particle sizes and expansive genomes. In this study, we collected water samples from 44 distinct locations along a river within Brazil's Pantanal biome. These samples were processed and subsequently inoculated into Vermamoeba vermiformis cultures. Observation of cytopathic effects led to the isolation of a novel virus, designated Naiavirus, distinguished by its unprecedented virion morphology and genomic organization. To elucidate the protein composition of Naiavirus particles, large-scale infections were conducted using 20 T175 flasks, each containing 10 million amoebae. Post-infection, viral particles were purified through two successive sucrose gradient centrifugations to achieve high purity. Proteomic analysis of these purified particles identified 254 associated viral proteins, a count exceeding those reported for all known giant viruses except for pandoraviruses. This dataset encompasses the raw mass spectrometry data, identification files, and associated metadata, offering valuable insights into the structural proteome of this unique giant virus.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:06:03.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Talita Hanchuk
SpeciesList	scientific name: Tupanvirus; NCBI TaxID: 2094720;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 04:46:24	ID requested
⏵ 1	2025-09-28 16:06:04	announced

Rodrigues M, Queiroz V, Arantes T, Limbor, ç, o H, Neiva B, Arias N, Machado T, Barcelos M, Cortines JR, Thiemann OH, Marques RE, Melo-Hanchuk TD, Hurtado Celis EL, Araujo JP, Reis E, Junior Alcantara LC, Santos CBC, Jivaji A, Rodrigues RAL, Aylward FO, Abrah, ã, o JS, Naiavirus: an enveloped giant virus with a pleomorphic, flexible tail. Nat Commun, 16(1):8306(2025) [pubmed]

10.1038/s41467-025-63463-6;

submitter keyword: Naiavirus, giant virus, proteome

Talita Diniz Melo Hanchuk
contact affiliation	CNPEM
contact email	talita.hanchuk@lnbio.cnpem.br
lab head
Talita Hanchuk
contact affiliation	Brazilian Center for Research in Energy and Materials (CNPEM)
contact email	talita.hanchuk@lnbio.cnpem.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD063493

PRIDE project URI

• PRIDE
  1. PXD063493
     1. Label: PRIDE project
     2. Name: Proteomic Characterization of Naiavirus