PXD063487

PXD063487 is an original dataset announced via ProteomeXchange.

Title	Plasma Proteomics Analysis of Human Host Response to Loa Loa, Validation
Description	Loiasis, caused by the filarial nematode Loa loa, imposes a significant burden of disease in endemic regions in West and Central Africa. Manifestations include microfilaria in the blood (MF) and migration of the adult eye worm (EW), with clinical presentations ranging from asymptomatic infections to life threatening organ involvement. The diagnostic gold standard, microscopic detection of microfilaria in the blood, remains difficult due to widely varying microfilaria counts, frequent amicrofilaraemia in patients with EW, and unreliable serological assays. By applying high-throughput plasma proteomics, we investigated the human host response in 274 patients with different L. loa disease states (INF), including EW (n=148), MF (n=42), or both (EWMF; n=84), compared to 136 loa-negative (LN) controls. Five proteins were elevated in INF compared to LN, including IGHG3, IGHG4, and LCP1. Four followed the trend from LN to EW to MF and EWMF, reflecting a more pronounced host response to higher microfilaria couMoMoreover, IGHG4 correlated positively with eosinophil and microfilarial counts underlining its role in chronic, parasitic infections. 63 plasma proteins exhibited notable differences depending on self-reported symptoms. The proteomic signature allowed for accurate classification of EW (AUROC = 0.73) and microfilaria positive (0.84) individuals. Together, L. loa infection leads to relevant alterations of the host plasma proteome, highlighting the importance of further in-depth research on this highly neglected parasitic disease. Here, we present additional experiments to validate IGHG4, IGHG3, LCP1, ACTBL2 and IGLV9-49 as proteomic biomarkers for Loa Loa Infection.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-14_08:41:26.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clemens Dierks
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 02:50:43	ID requested
⏵ 1	2025-07-14 08:41:26	announced

10.1093/INFDIS/JIAF344;

submitter keyword: MS, Plasma, Proteomics,NTD

Markus Ralser
contact affiliation	Department of Biochemistry, Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Berlin, Germany Berlin Institute of Health (BIH) at Charité Universitätsmedizin Berlin, Berlin, Germany The Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Roosevelt Dr, Headington, Oxford OX3 7BN, UK
contact email	markus.ralser@charite.de
lab head
Clemens Dierks
contact affiliation	Institute of Biochemistry, Charité Universitaetsmedizin Berlin, Germany
contact email	clemens.dierks@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063487

PRIDE project URI

• PRIDE
  1. PXD063487
     1. Label: PRIDE project
     2. Name: Plasma Proteomics Analysis of Human Host Response to Loa Loa, Validation