PXD063470

PXD063470 is an original dataset announced via ProteomeXchange.

Title	Multisite phosphorylation of intrinsically disordered region of Dishevelled
Description	When WNT binds to its receptor Frizzled (FZD) to activate the signaling, Dishevelled (DVL), the central component of Wnt signaling, is phosphorylated by redundant Casein kinase 1 (CK1) isoforms ε or δ, to transduce the signal downstream. Although the basic principles of Wnt signal transduction are known, the mechanistic aspects of DVL phosphorylation remain elusive. Here we identify Wnt-dependent multisite phosphorylation of a large and conserved serine/threonine (S/T) cluster within intrinsically disordered region (IDR), located between the PDZ and DEP domains. The detailed analysis of the phosphorylation and its effect on the flanking domains was performed with the construct containing both domains PDZ and DEP flanking the IDR (hDVL3 PDZ-DEP, aa 243-496).
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_00:02:12.547.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	monohydroxylated residue
Instrument	nanoACQUITY UPLC with HDX Technology; Synapt G2 HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 23:55:32	ID requested
⏵ 1	2026-04-07 00:02:13	announced

Dataset with its publication pending

submitter keyword: Dishevelled, phosphorylation, HDX-MS

Cherine Bechara
contact affiliation	University of Montpellier, France
contact email	Cherine.bechara@umontpellier.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063470
     1. Label: PRIDE project
     2. Name: Multisite phosphorylation of intrinsically disordered region of Dishevelled