PXD063463 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome-wide Analysis of Palmitoylated Proteins in Macrophages Reveals Novel Insights into Early Immune Signaling |
| Description | To understand protein S-palmitoylation's role in macrophage reprogramming, dynamic changes were comprehensively mapped in immortalized mouse macrophages during early LPS stimulation using acyl-biotin exchange (ABE) proteomics enhanced with multi-protease digestion. This approach identified 2613 putative S-palmitoylated proteins, including 587 novel mouse candidates, many involved in immunity. Following 30 minutes of LPS exposure, 648 proteins showed altered palmitoylation, indicating dynamic regulation linked functionally to immune signaling, transport, cellular organization, and metabolism. This research provides a key resource on the macrophage palmitoylome's remodeling during immune activation, uncovering new potential regulatory targets. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-20_07:48:33.539.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Aleksandra Nita-Lazar |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | palmitoylated residue |
| Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-29 13:12:12 | ID requested | |
| ⏵ 1 | 2026-08-20 07:48:34 | announced | |
Publication List
| 10.1002/pmic.70100; |
| Kim H, Issara-Amphorn J, Yoon SH, Banerjee A, Nita-Lazar A, Proteome-Wide Analysis of Palmitoylated Proteins in Macrophages Reveals Novel Insights Into Early Immune Signaling. Proteomics, 26(8):69-80(2026) [pubmed] |
Keyword List
| submitter keyword: Macrophage, Acyl-biotin exchange (ABE),Palmitoylation, Immune signaling, S-acylation, Post-translational modification, Lipopolysaccharide (LPS), Multi-protease digestion, Proteomics |
Contact List
| Aleksandra Nita-Lazar |
|---|
| contact affiliation | NIAID, NIH |
| contact email | nitalazarau@niaid.nih.gov |
| lab head | |
| Aleksandra Nita-Lazar |
|---|
| contact affiliation | NIAID |
| contact email | nitalazarau@niaid.nih.gov |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD063463 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063463
- Label: PRIDE project
- Name: Proteome-wide Analysis of Palmitoylated Proteins in Macrophages Reveals Novel Insights into Early Immune Signaling
