PXD063455

PXD063455 is an original dataset announced via ProteomeXchange.

Title	Discovery of an Exquisitely Selective WDR5 Chemical Probe Accelerated by a High-Quality DEL-ML Hit
Description	A chemoproteomics experiment was performed to investigate the selectivity of the WDR5 targeting compound LH168. Therefore, a biotinylated adduct of LH168, namely LH205, was used to enrich potential target proteins of LH168. The addition of LH168 in excess competed only the interaction with LH168, demonstrating a proteome wide selectivity of LH168 for WDR5.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_18:00:50.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Gstaiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 08:55:58	ID requested
⏵ 1	2025-09-14 18:00:51	announced

Hoffmann L, Lenz C, Farges F, Kimani SW, Dopfer J, Keller S, Schwalm MP, Holzmann H, Kraemer A, Dong A, Li F, Chau I, Halabelian L, Gstaiger M, M, ü, ller S, Knapp S, N, ě, mec V, Discovery of an exquisitely selective WDR5 chemical probe accelerated by a high-quality DEL-ML Hit. RSC Chem Biol, 6(10):1585-1594(2025) [pubmed]

10.1039/d5cb00109a;

submitter keyword: chemoproteomics, WDR5

Matthias Gstaiger
contact affiliation	ETH Zürich Institute of Molecular Systems Biology Otto-Stern-Weg 3 8093 Zürich Switzerland
contact email	gstaigem@ethz.ch
lab head
Matthias Gstaiger
contact affiliation	Institute of Molecular Systems Biology, ETH
contact email	gstaigem@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD063455

PRIDE project URI

• PRIDE
  1. PXD063455
     1. Label: PRIDE project
     2. Name: Discovery of an Exquisitely Selective WDR5 Chemical Probe Accelerated by a High-Quality DEL-ML Hit