PXD063449

PXD063449 is an original dataset announced via ProteomeXchange.

Title	Iron regulatory pathways differentially expressed during Madurella mycetomatis grain development in Galleria mellonella
Description	Mycetoma is a chronic granulomatous infection of the subcutaneous tissue, most often caused by the fungal pathogen Madurella mycetomatis. Characteristic of the infection is the formation of grains. However, knowledge of the function and formation of the grain is limited. To map the processes leading to M. mycetomatis grain formation, we used a Galleria mellonella larvae infection model and transcriptomic profiling. G. mellonella larvae were infected with M. mycetomatis and after 4h, 24h, 72h and 168h post-inoculation, RNA was extracted from larval content and sequenced. Differential Gene Expression analysis revealed that 3,498 G. mellonella and 136 M. mycetomatis genes were differentially expressed during infection. Among these genes, genes related to iron transport were highly expressed by both G. mellonella (transferrin and ferritin) and M. mycetomatis (SidA, SidD and SidI). LC-MS/MS analysis of M. mycetomatis cultured under iron-limiting conditions revealed the presence of SidA and SidD orthologs and concurrent RP-HPLC and LC-MS identified a singly charged, putative siderophore in culture supernatant. M. mycetomatis could also obtain iron from holoferritin. The identification of the importance of iron acquisition during grain formation can be exploited as a potential novel diagnostic and therapeutic strategy for mycetoma.
HostingRepository	PRIDE
AnnounceDate	2025-05-15
AnnouncementXML	Submission_2025-05-15_06:33:51.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	cathal murphy
SpeciesList	scientific name: Madurella mycetomatis; NCBI TaxID: 100816;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 07:28:25	ID requested
⏵ 1	2025-05-15 06:33:52	announced

10.1101/2022.12.20.520897;

submitter keyword: mycetomatis grain,Eumycetoma, transcriptomics, siderophores, Galleria mellonella

Sean Doyle
contact affiliation	Department of Biology, Maynooth University
contact email	sean.doyle@mu.ie
lab head
cathal murphy
contact affiliation	Maynooth University
contact email	cathalmurphy0@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063449

PRIDE project URI

• PRIDE
  1. PXD063449
     1. Label: PRIDE project
     2. Name: Iron regulatory pathways differentially expressed during Madurella mycetomatis grain development in Galleria mellonella