PXD063447

PXD063447 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Stress specific SCE1-interactome characterised by IP-MS analysis
Description	The AtSCE1-mVenus was immunoprecipitated using anti-GFP magnetic beads from Arbidopsis root cultures upon control, salt, mannitol and flg22 treatments. As a negative control, root tissues from pMDS1-VAM3 and pMDS1-SHR were utilized to account for non-specific binding to GFP. The eluate sapmles were digested using trypsin/Lys-C in gel and further LC-MS/MS analysis was performed using Thermo Fisher Q Exactive Mass Spectrometer.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_16:28:45.745.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063447
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kishor D Ingole
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 07:12:55	ID requested
⏵ 1	2025-09-07 16:28:46	announced

Publication List

Banda J, Ghosh S, Roy D, Ingole KD, Clark L, Sharma E, Kakkunath S, Sue-Ob K, Bhosale R, Band L, Ghosh S, Wells D, Atkinson J, Provart NJ, Bennett MJ, Lilley KS, Jones A, De Lucas M, Bishopp A, Sadanandom A, Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote. Sci Adv, 11(35):eadw9153(2025) [pubmed]
10.6019/PXD063447;
10.1126/sciadv.adw9153;

Banda J, Ghosh S, Roy D, Ingole KD, Clark L, Sharma E, Kakkunath S, Sue-Ob K, Bhosale R, Band L, Ghosh S, Wells D, Atkinson J, Provart NJ, Bennett MJ, Lilley KS, Jones A, De Lucas M, Bishopp A, Sadanandom A, Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote. Sci Adv, 11(35):eadw9153(2025) [pubmed]

10.6019/PXD063447;

10.1126/sciadv.adw9153;

Keyword List

submitter keyword: SUMOylation, IP-MS,At-SCE1-interactome, Arabidopsis thaliana root cultures

submitter keyword: SUMOylation, IP-MS,At-SCE1-interactome, Arabidopsis thaliana root cultures

Contact List

Kathryn S. Lilley
contact affiliation	Professor, Department of Biochemistry, University of Cambridge, UK.
contact email	ksl23@cam.ac.uk
lab head
Kishor D Ingole
contact affiliation	Postdoctoral Research Associate
contact email	kdi20@cam.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD063447
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD063447

PRIDE project URI

Repository Record List

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