PXD063444

PXD063444 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals differential diagnosis biomarkers between sepsis and hemophagocytic syndrome
Description	Hemophagocytic Lymphohistiocytosis (HLH) shares many clinical features with sepsis. To improve HLH diagnosis and its differential diagnosis with sepsis, serum proteomic analyses of healthy donors, HLH and septic patients were performed. In this study, 24 individuals were enrolled in a label free MS/MS spectrometry analysis. Proteomic results revealed 537 differentially expressed proteins (DEP) between HLH and sepsis, 471 DEP between HLH and healthy donors and 37 DEP between sepsis and healthy donors. After protein identification and quantification, results were subjected to functional analysis to study impaired processes in each condition. These functional analyses showed that, apart from inflammation and lipid metabolism, the proteostasis network was deeply impaired in HLH condition. Considering this information, each protein fold changes, their function, and other previously published related to DEP, six proteins were validated by ELISA in a larger cohort of patients and healthy donors. Our results revealed that sCD300a, sCD300b and sCD25 could be specific serum biomarkers for HLH diagnosis. SAA-1 and LRG1 might be useful biomarkers for differential diagnosis between sepsis and HLH, and PSMB1 showed promising results for HLH specific and differential diagnosis. In this sense, PSMB1 was an interesting biomarker and its elevation in HLH patients may reflect an intensified demand for protein turnover, possibly driven by a higher activation of immunoproteasome. These insights contribute to expand our understanding of HLH pathophysiology in new pathways and highlight innovative therapeutic intervention such as Bortezomib and other next-generation inhibitors designed to modulate immunoproteasome activity
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_17:50:55.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 06:24:32	ID requested
⏵ 1	2026-01-04 17:50:56	announced

Martin-Pestana D, Azkargorta M, Pilar-Orive FJ, Redondo S, Urrutia J, Calvo C, Elortza F, Astigarraga I, Garcia-Obregon S, Proteomics Reveals Differential Diagnosis Biomarkers Between Sepsis and Hemophagocytic Syndrome. Biomedicines, 13(12):(2025) [pubmed]

10.3390/biomedicines13123113;

submitter keyword: hemophagocytic syndrome,Sepsis, label free

Felix Elortza
contact affiliation	Proteomics Platform CIC bioGUNE Bizkaia Tech. Park Build. 800 Derio 48160 Spain
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063444

PRIDE project URI

• PRIDE
  1. PXD063444
     1. Label: PRIDE project
     2. Name: Proteomics reveals differential diagnosis biomarkers between sepsis and hemophagocytic syndrome